Protective effect of Syzygium cumini against pesticide-induced cardiotoxicity
Protective effect of Syzygium cumini against pesticide-induced cardiotoxicity
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DOI：
10.1007/s11356-014-2684-3
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发表时间：
2014-07-01
影响因子：
5.8
通讯作者：
Rani, Vibha
中科院分区：
文献类型：
--
作者：
Atale, Neha;Gupta, Khushboo;Rani, Vibha
农药的毒性是一个严重的问题，在世界范围内导致了大量的疾病。有机磷农药马拉硫磷已引起许多中毒事件，如心脏表现。本研究旨在评价蒲桃对马拉硫磷心脏毒性的影响。采用MTT法对马拉硫磷和姜黄素、表儿茶素、没食子酸、二丁基羟基甲苯等多酚类物质的剂量进行了优化。通过不同的技术分析核畸形、ROS产生和细胞外基质成分的完整性。S.研究了一种富含没食子酸的天然抗氧化剂--孜然果浆甲醇提取物（MPE）对马拉硫磷（Malathion）毒性的影响。还分析了核畸形、ROS产生和细胞外基质组分的完整性。发现20微克/毫升LD 50剂量的马拉硫磷在H9 C2细胞系中引起应激介导的反应。在所有的多酚类物质中，没食子酸表现出最显着的抗应激保护。富没食子酸甲醇S.与乙醇浆提取物和水浆提取物相比，孜然芹浆提取物（MPE）对DPPH、ABTS、NO、H2 O2和超氧离子的最大抑制分别为59.76%+/-0.05，81.61% +/-1.37，73.33% +/-1.33，77.19% +/-2.38和64.19% +/-1.43。我们的研究表明S.枯否醇MPE具有保护心肌细胞免受马拉硫磷介导的氧化应激的能力。
Pesticide-induced toxicity is a serious issue which has resulted in plethora of diseases all over the world. The organophosphate pesticide malathion has caused many incidents of poisoning such as cardiac manifestations. The present study was designed to evaluate the effect of Syzygium cumini on malathion-induced cardiotoxicity. Dose optimization of malathion and polyphenols such as curcumin, (-)-epicatechin, gallic acid, butylated hydroxyl toluene, etc. was done by MTT cell proliferation assay. Nuclear deformities, ROS production, and integrity of extra cellular matrix components were analyzed by different techniques. S. cumini methanolic pulp extract (MPE), a naturally derived gallic acid-enriched antioxidant was taken to study its effect on malathion-induced toxicity. Nuclear deformities, ROS production, and integrity of extra cellular matrix components were also analyzed. Twenty micrograms per milliliter LD50 dose of malathion was found to cause stress-mediated responses in H9C2 cell line. Among all the polyphenols, gallic acid showed the most significant protection against stress. Gallic acid-enriched methanolic S. cumini pulp extract (MPE) showed 59.76 % +/- 0.05, 81.61% +/- 1.37, 73.33% +/- 1.33, 77.19% +/- 2.38 and 64.19% +/- 1.43 maximum inhibition for DPPH, ABTS, NO, H2O2 and superoxide ion, respectively, as compared to ethanolic pulp extract and aqueous pulp extract. Our study suggests that S. cumini MPE has the ability to protect against the malathion-mediated oxidative stress in cardiac myocytes.