Proinflammatory Cytokine Profiling of Tears from Dry Eye Patients by Means of Antibody Microarrays
Proinflammatory Cytokine Profiling of Tears from Dry Eye Patients by Means of Antibody Microarrays
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DOI：
10.1167/iovs.11-7266
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发表时间：
2011-09-01
影响因子：
4.4
通讯作者：
Grus, Franz H.
中科院分区：
文献类型：
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作者：
Boehm, Nils;Riechardt, Aline I.;Grus, Franz H.
目的.在干燥性角结膜炎的发病机制中，免疫过程被认为起着重要作用。然而，病理机制的确切细节仍然未知。本研究分析了不同类型干眼症患者泪液中促炎细胞因子的表达模式。143名受试者被细分为健康对照组（CTRL，n = 38）、缺水性干眼症患者（DRYaq，n = 35）、脂质层改变患者（DRYlip，n = 36）和两者结合的患者（DRYaplip，n = 34）。蛋白质的表达模式（例如，使用先进的抗体微阵列方法检测IL-1 β、IL-6、ITNF-α和IFN-γ。干眼患者的细胞因子水平与健康对照组相比，检测到几个高度显着的差异。DRYaq患者或DRYaplip患者的大多数测试蛋白质水平升高。例如，在DRYaq患者中发现IL-1 β升高2.4倍，在DRYaqlip患者中升高2.75倍（均P < 8.00E-6）。DRYlip患者和健康对照组中检测到的蛋白质量仅显示出极小的差异（所有蛋白质的增加/减少倍数> 1.2; P > 5.00E-1）。健康对照和DRYlip患者的特征之间的相似性证明了这种干眼亚型的病理机制是基于炎症以外的机制的假设，而DRYaq患者似乎是这种情况。（Invest Ophthalmol维斯科学。2011;52：7725-7730）DOI：10.1167/iovs.11-7266
PURPOSE. In the pathogenesis of keratoconjunctivitis sicca, immune processes are thought to play an important role. However, the exact details of the pathomechanisms are still unknown. In this study, the expression patterns of proinflammatory cytokines in the tears of patients with different subtypes of dry eye were analyzed.METHODS. One hundred forty-three subjects subdivided into healthy controls (CTRL, n = 38), patients with aqueous-deficient dry eye (DRYaq, n = 35), patients with changes of the lipid layer (DRYlip, n = 36), and patients with a combination of both (DRYaplip, n = 34) were examined. Expression patterns of proteins (e.g., IL-1 beta, IL-6, ITNF-alpha, and IFN-gamma) were examined using an advanced antibody microarray approach.RESULTS. Several highly significant differences in the cytokine levels of dry eye patients compared with healthy controls were detected. Patients with DRYaq or those with DRYaplip showed elevated levels for most of the tested proteins. For example, IL-1 beta was found to be elevated 2.4-fold in DRYaq patients and 2.75-fold in DRYaqlip patients (both P < 8.00E-6). The detected amounts of protein in DRYlip patients and in healthy controls showed only minimal differences (fold increase/decrease for all proteins > 1.2; P > 5.00E-1).CONCLUSIONS. The similarity between the profiles of healthy controls and DRYlip patients justifies the assumption that the pathomechanism of this dry eye subtype is based on mechanisms other than inflammation, whereas it seems to be the case for DRYaq patients. (Invest Ophthalmol Vis Sci. 2011;52:7725-7730) DOI:10.1167/iovs.11-7266