EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition during Cold Stress in Arabidopsis
EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition during Cold Stress in Arabidopsis
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DOI：
10.1093/pcp/pcaa139
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发表时间：
2021-01-01
影响因子：
4.9
通讯作者：
Fujii, Hiroaki
中科院分区：
文献类型：
--
作者：
Barajas-Lopez, Juan de Dios;Tiwari, Arjun;Fujii, Hiroaki
植物通过改变生理活动如基因表达来适应不利条件。虽然以前的研究已经确定了多个应激诱导基因，但许多基因在应激反应中的功能仍然不清楚。ERD 7（Early Response TO Dehydration 7）是脱水后的一种早期反应。在这里，我们表明ERD7在植物胁迫反应和发育中起着至关重要的作用。在拟南芥中，ERD7蛋白在各种胁迫条件下积累，包括暴露于低温。一个三重突变体的拟南芥缺乏ERD7和两个密切相关的同源物具有胚胎致死表型，而一个突变体缺乏这两个同源物和一个ERD7等位基因有相对圆叶，表明ERD7基因家族在发展中具有重要作用。此外，ERD7家族在应激反应中的重要性通过突变株系对冷应激的易感性得到证明。ERD7蛋白被发现结合到几个，但不是所有的，带负电荷的磷脂，并与膜。相对于野生型，突变株系中的脂质组分和冷诱导的PIP2减少发生了改变。此外，来自突变株系的膜具有降低的流动性。综上所述，ERD7及其同源物在植物逆境响应和发育中起重要作用，并与膜脂组成的改变有关。
Plants adjust to unfavorable conditions by altering physiological activities, such as gene expression. Although previous studies have identified multiple stress-induced genes, the function of many genes during the stress responses remains unclear. Expression of ERD7 (EARLY RESPONSE TO DEHYDRATION 7) is induced in response to dehydration. Here, we show that ERD7 plays essential roles in both plant stress responses and development. In Arabidopsis, ERD7 protein accumulated under various stress conditions, including exposure to low temperature. A triple mutant of Arabidopsis lacking ERD7 and two closely related homologs had an embryonic lethal phenotype, whereas a mutant lacking the two homologs and one ERD7 allele had relatively round leaves, indicating that the ERD7 gene family has essential roles in development. Moreover, the importance of the ERD7 family in stress responses was evidenced by the susceptibility of the mutant lines to cold stress. ERD7 protein was found to bind to several, but not all, negatively charged phospholipids and was associated with membranes. Lipid components and cold-induced reduction in PIP2 in the mutant line were altered relative to wild type. Furthermore, membranes from the mutant line had reduced fluidity. Taken together, ERD7 and its homologs are important for plant stress responses and development and associated with the modification in membrane lipid composition.