Brucella microti: the genome sequence of an emerging pathogen
Brucella microti: the genome sequence of an emerging pathogen
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DOI：
10.1186/1471-2164-10-352
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发表时间：
2009-08-04
期刊：
影响因子：
4.4
通讯作者：
Scholz, Holger C.
中科院分区：
文献类型：
--
作者：
Audic, Stephane;Lescot, Magali;Scholz, Holger C.
背景资料：使用焦磷酸测序和常规桑格测序的组合，最近描述的新的布鲁氏菌物种，布氏菌，全基因组序列进行了测定。B。田鼠是阿尔法变形菌纲中布鲁氏菌属的成员，该菌由医学上重要的高致病性兼性胞内细菌组成。与其他布鲁氏菌属不同，B. Microti是一种快速生长和生物化学活性非常高的微生物，其表型更类似于一种兼性人类病原体苍白杆菌。B的非典型表型。microti促使我们寻找基因组的差异相比，其他布鲁氏菌物种，并寻找与Ochrobactrum.Results的相似之处：基因组由两个环状染色体的2，117，050和1，220，319碱基对。出乎意料的是，我们发现B的基因组序列。microti与猪布鲁氏杆菌1330几乎相同，在比对区域中的总体序列同一性为99.84%。两个基因组之间最显著的结构差异是仅存在于B中的噬菌体相关的11，742个碱基对插入物。显微镜然而，该插入物不太可能具有任何表型后果。B之间仅共享四个蛋白质编码基因。microti和Ochrobactrum anthropi，但在其他测序布鲁氏菌受损。B之间最明显的区别。在23S核糖体RNA基因的序列中发现了田鼠和其他布鲁氏菌物种。这种不寻常的变化可能具有多效性效应，并解释了B的快速生长。结论：与表型分析结果相反，B. microti与布氏杆菌属已知种的相似度较高，与O.人类学B之间基因含量的差异有多大。microti和B. SUIS 1330可能导致非常不同的表型仍有待阐明。这一意外的发现将使布鲁氏菌属中鉴定毒力决定因子的任务复杂化。B的基因组序列。microti将作为差异表达分析和互补研究的模型。我们的研究结果也提出了一些关注的重要性，在细菌物种的定义表型性状。
Background: Using a combination of pyrosequencing and conventional Sanger sequencing, the complete genome sequence of the recently described novel Brucella species, Brucella microti, was determined. B. microti is a member of the genus Brucella within the Alphaproteobacteria, which consists of medically important highly pathogenic facultative intracellular bacteria. In contrast to all other Brucella species, B. microti is a fast growing and biochemically very active microorganism with a phenotype more similar to that of Ochrobactrum, a facultative human pathogen. The atypical phenotype of B. microti prompted us to look for genomic differences compared to other Brucella species and to look for similarities with Ochrobactrum.Results: The genome is composed of two circular chromosomes of 2,117,050 and 1,220,319 base pairs. Unexpectedly, we found that the genome sequence of B. microti is almost identical to that of Brucella suis 1330 with an overall sequence identity of 99.84% in aligned regions. The most significant structural difference between the two genomes is a bacteriophage-related 11,742 base pairs insert only present in B. microti. However, this insert is unlikely to have any phenotypical consequence. Only four protein coding genes are shared between B. microti and Ochrobactrum anthropi but impaired in other sequenced Brucella. The most noticeable difference between B. microti and other Brucella species was found in the sequence of the 23S ribosomal RNA gene. This unusual variation could have pleiotropic effects and explain the fast growth of B. microti.Conclusion: Contrary to expectations from the phenotypic analysis, the genome sequence of B. microti is highly similar to that of known Brucella species, and is remotely related to the one of O. anthropi. How the few differences in gene content between B. microti and B. suis 1330 could result in vastly different phenotypes remains to be elucidated. This unexpected finding will complicate the task of identifying virulence determinants in the Brucella genus. The genome sequence of B. microti will serve as a model for differential expression analysis and complementation studies. Our results also raise some concerns about the importance given to phenotypical traits in the definition of bacterial species.