A Simple Method for the Determination of Serum Free Insulin Levels in Insulin-treated Patients
A Simple Method for the Determination of Serum Free Insulin Levels in Insulin-treated Patients
复制标题
测定胰岛素治疗患者血清游离胰岛素水平的简单方法
作者：
S. Nakagawa;H. Nakayama;Takashi Sasaki;K. Yoshino;Yune Ying Yu;K. Shinozaki;S. Aoki;K. Mashimo
本文介绍了一种测定经胰岛素治疗的糖尿病患者血清中游离活性胰岛素的方法。这涉及用聚乙二醇提取游离胰岛素后的放射免疫测定。用冷胰岛素进行的恢复试验显示，游离胰岛素恢复了73%，结合胰岛素没有恢复。患者的空腹游离胰岛素水平略低于正常人;例外情况是一些并发症患者预期会导致外周组织水平的胰岛素抵抗。游离胰岛素水平与总胰岛素水平、抗体滴度、胰岛素需求或胰岛素治疗条件无关。在胰岛素治疗的患者中，在葡萄糖负荷后观察到胰岛素非常轻微的增加，但在胰岛素自身免疫综合征患者中观察到游离胰岛素水平显著增加，随后总胰岛素水平过度增加。游离胰岛素的昼夜变化表明了这一部分的动态状态及其对确定胰岛素控制糖尿病的有用性。
A method for the determination of free, active insulin in the sera of insulin-treated diabetics is described. This involved radioimmunoassay after extraction of free insulin with polyethylene glycol. Recovery tests with cold insulin showed 73 per cent recovery of free insulin and no recovery of bound insulin. The fasting free insulin levels were slightly lower in the patients than in normal persons; exceptions were some patients with complications expected to cause insulin resistance at the peripheral tissue level. Free insulin levels did not correlate with total insulin levels, antibody titers, insulin requirements or conditions of insulin treatment. A very slight increase of insulin was observed after glucose loading in insulin-treated patients, but a marked increase of the free insulin level followed by an exaggerated increase in the total insulin level was observed in a patient with the insulin autoimmune syndrome. The diurnal changes of the free insulin suggested the dynamic states of this fraction and its usefulness for determining control of diabetes with insulin.