EBER in situ hybridization for Epstein-Barr virus.
EBER in situ hybridization for Epstein-Barr virus.
复制标题
DOI：
10.1007/978-1-62703-357-2_16
复制
发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Chen, Yuan-Yuan
中科院分区：
文献类型：
--
作者：
Weiss, Lawrence M;Chen, Yuan-Yuan
EB病毒编码区（EBER）原位杂交是检测组织切片中EB病毒（EBV）的首选方法。由于潜伏感染细胞中存在大量EBER拷贝，因此可以使用非同位素方法。阳性研究显示EBV感染细胞的细胞核染色，染色质突出，通常不包括核仁。假阴性结果通常是组织中RNA降解的结果，这一发现可以通过使用聚T探针作为RNA保存的对照来检测。
Epstein-Barr encoding region (EBER) in situ hybridization is the methodology of choice for the detection of the Epstein-Barr virus (EBV) in tissue sections. Because of the large numbers of copies of EBERs present in latently infected cells, non-isotopic methods can be used. Positive studies show staining in the nuclei of the EBV-infected cells, accentuating the chromatin and often excluding the nucleolus. False-negative results are most often the result of RNA degradation in the tissues, a finding that may be detected through the use of a polyT probe as a control for RNA preservation.