Volatile Organic Metabolites Identify Patients with Mesangial Proliferative Glomerulonephritis, IgA Nephropathy and Normal Controls.
Volatile Organic Metabolites Identify Patients with Mesangial Proliferative Glomerulonephritis, IgA Nephropathy and Normal Controls.
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挥发性有机代谢物可识别系膜增生性肾小球肾炎、IgA 肾病和正常对照患者
DOI：
10.1038/srep14744
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发表时间：
2015-10-07
影响因子：
4.6
通讯作者：
Li E
中科院分区：
文献类型：
--
作者：
Wang C;Feng Y;Wang M;Pi X;Tong H;Wang Y;Zhu L;Li E
近年来，肾脏疾病的尿液挥发性有机化合物(VOCs)分析引起了人们极大的科学兴趣，尿液代谢物分析已经应用于多种疾病。收集15例系膜增生性肾小球肾炎(MsPGN)患者、21例IgA肾病(IgAN)患者和15例健康人的尿液。采用固相微萃取-气相色谱-质谱法(SPME-GC-MS)分析尿液中的代谢物。采用主成分分析(PCA)和正交偏最小二乘判别分析(OPLSDA)对最终数据进行处理。MsPGN组有5种代谢产物明显高于正常对照组(P <； )，而IgA肾病组有3种代谢物高于正常对照组(P <； 0.05)。此外，IgA肾病组的5种代谢产物较MSPGN组显著升高(P <； 0.0 5)。这5种代谢产物可能是鉴别MsPGN和IgAN的特异性生物标志物。尿VOCs分析作为一种诊断工具具有潜在的临床应用价值。
Urinary volatile organic compounds (VOCs) analysis for kidney diseases has attracted a large amount of scientific interest recently and urinary metabolite analysis has already been applied to many diseases. Urine was collected from 15 mesangial proliferative glomerulonephritis (MsPGN) patients, 21 IgA nephropathy (IgAN) patients and 15 healthy controls. Solid phase microextraction–chromatography– mass spectrometry (SPME-GC-MS) was used to analyse the urinary metabolites. The statistical methods principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLSDA) were performed to process the final data. Five metabolites were significantly greater in the group of MsPGN patients than in the normal control group (P < 0.05) while three metabolites were found at increased levels in the group of IgAN patients compared with the normal controls (P < 0.05). In addition, five metabolites were significantly increased in the group of IgAN patients compared with the MsPGN patients (P < 0.05). These five metabolites may be specific biomarkers for distinguishing between MsPGN and IgAN. The analysis of urinary VOCs appears to have potential clinical applications as a diagnostic tool.