The first report of transmission of a phytoplasma associated with sesame phyllody by Orosius albicinctus in Iran
The first report of transmission of a phytoplasma associated with sesame phyllody by Orosius albicinctus in Iran
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DOI：
10.1071/dn07016
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发表时间：
2007-12-01
影响因子：
0.9
通讯作者：
Rahimian, H.
中科院分区：
文献类型：
--
作者：
Esmailzadeh-Hosseini, S. A.;Mirzaie, A.;Rahimian, H.
2003- 2005年期间，在伊朗亚兹德省的芝麻田进行了植原体调查。采集了叶蝉Orosius albicinctus，发现其含有植原体。在温室条件下进行了传输测试。指示植物为苜蓿、油菜、甜菜、芝麻、萝卜、长春花和水芹。应用植原体通用引物建立了巢式聚合酶链反应技术，对指示植物和叶蝉中的植原体进行了检测。植原体特征的1.2-kb的16 S rDNA片段的扩增证实，芝麻，菜和甜菜植株被植原体感染。用5种内切酶对扩增的DNA片段进行RFLP分析，确定该植原体属于16 SrII组（花生丛枝植原体）。这是首次报道由O.伊朗的白纹伊蚊
During 2003-05, a survey for phytoplasmas was carried out in sesame fields in Yazd province (Iran). The leafhopper Orosius albicinctus was collected and found to contain a phytoplasma. Transmission tests were performed under greenhouse conditions. The indicator plants were alfalfa, rapeseed, garden beet, sesame, radish, periwinkle and garden cress. A nested polymerase chain reaction using phytoplasma universal primers was used to detect phytoplasmas in indicator plants and leafhoppers. Amplification of a phytoplasma-characteristic 1.2-kb 16 S rDNA fragment confirmed that sesame, garden cress and garden beet plants were infected by the phytoplasma. On the basis of RFLP analysis of the amplified DNA fragments using five endonucleases, the detected phytoplasma belonged to the 16SrII group (peanut witches' broom phytoplasma). This is the first report of transmission of a phytoplasma associated with sesame phyllody by O. albicinctus in Iran.