DNA-catalyzed efficient production of single-stranded DNA nanostructures
DNA-catalyzed efficient production of single-stranded DNA nanostructures
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DNA 催化高效生产单链 DNA 纳米结构
DOI：
10.1016/j.chempr.2020.12.001
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发表时间：
2021-04-08
期刊：
影响因子：
23.5
通讯作者：
Gu, Hongzhou
中科院分区：
文献类型：
--
作者：
Jia, Youli;Chen, Liman;Gu, Hongzhou
单链DNA纳米结构（DNs）是由碱基单链DNA的单分子折叠而成的，代表了一种适合生物材料和生物医学应用的新型DNA结构。在这里，使用DNA切割DNA酶（脱氧核酶），我们提出了一种高效和经济地生产毫克ssdn的策略。我们设计了带有自裂脱氧核酶的ssdn“基因”，并以单链形式扩增了这些基因。在自动处理后，产生的扩增子释放出千万亿份自我折叠成所需结构的ssDNA拷贝。通过设计反式作用脱氧核酶，我们发现几种不同形状的ssdn可以被编码和扩增，同时从扩增子中可控地释放特定形状的ssdn，从而提供了一种方便而经济的方法，可以安全地将纳米结构的“遗传密码”存储在DNA上，以便根据需要选择性地大规模生产。我们证明了ssdn辅助脂质体分选的能力，并期望该方法将促进ssdn在广泛领域的应用。
Single-stranded (ss) DNA nanostructures (DNs), created by unimolecular folding of kilo-based ssDNA, represent a new class of DNA constructs suitable for biomaterial and biomedical applications. Here, using DNA-cleaving DNA enzymes (deoxyribozymes), we present a strategy for the efficient and cost-effective production of milligrams of ssDNs. We designed "genes'' of ssDNs flanked by self-cleaving deoxyribozymes and amplified the genes in the single-stranded form. Upon autoprocessing, the resulting amplicons release a quadrillion copies of the ssDNA that self-folds into the desired structures. By engineering trans-acting deoxyribozymes, we showed that several ssDNs of different shapes can be encoded and amplified in tandem with controllable release of specific shapes from the amplicons, thereby providing a convenient yet economical way to safely store "genetic codes'' of nanostructures on DNA for selective mass production on demand. We demonstrated ssDNs' ability to assist liposome sorting and expect the approach will promote the development of ssDNs' applications in broad areas.