Potentiometric study of cytochrome c1aa3 from Thermus thermophilus.

Potentiometric study of cytochrome c1aa3 from Thermus thermophilus.
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嗜热栖热菌细胞色素 c1aa3 的电位研究。

DOI:
10.1016/0162-0134(85)85036-4
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发表时间:
1985
影响因子:
3.9
通讯作者:
Fee,JA
Fee,JA
中科院分区:
生物学2区
文献类型:
--
作者:
Yoshida,T;Fee,JA

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我们研究了来自嗜热栖热菌的细胞色素c1aa3复合物的氧化还原行为。在电位滴定中,细胞色素c表现为独立中心,n= 1且E= 205 mV (NHE)。假设各个中心在本实验中独立平衡,603 nm 吸收带的变化已分解为两个分量:细胞色素a(n = 1,Em = 270 mV,60%光谱贡献)和细胞色素a3(n = 2,Em = 360 mV,40%光谱贡献)。 then=2过程归因于细胞色素3和CuB之间的强化学耦合。还用 NADH 和 PMS 的混合物滴定该酶,根据电位滴定获得的平衡常数,结果显示不符合分子内平衡模型。这表明复合物内的构象平衡可以控制细胞色素aanda3之间的电子转移。
We have examined the redox behavior of the cytochromec1aa3complex fromThermus thermophilus. In potentiometric titrations the cytochromecbehaves as an independent center havingn= 1 andE= 205 mV (NHE). Under the assumption that the individual centers equilibrate independently in this experiment, changes in the absorption band at 603 nm have been resolved into two components: cytochromea(n= 1,Em= 270 mV, 60% spectral contribution) and cytochromea3(n= 2,Em= 360 mV, 40% spectral contribution). Then= 2 process was attributed to strong chemical coupling between cytochromea3and CuB. The enzyme was also titrated with a mixture of NADH and PMS, and the results are shown not to conform to a model of intramolecular equilibrium according to the equilibrium constants obtained from the potentiometric titration. It is suggested that a conformational equilibrium within the complex may control electron transfer between cytochromesaanda3.
DOI: --
发表时间: 1981
期刊: The Journal of biological chemistry
影响因子: --
作者:
Bull,C;Ballou,DP;Otsuka,S
通讯作者: Otsuka,S
细胞色素氧化酶和细胞色素 c 之间相互作用的动力学特征。
DOI: --
发表时间: 1982
期刊: The Journal of biological chemistry
影响因子: --
作者:
Antalis,TM;Palmer,G
通讯作者: Palmer,G
细胞色素氧化酶的结构特点及反应机制:铁和铜X射线吸收精细结构。
DOI: 10.1016/s0006-3495(81)84863-1
发表时间: 1981
影响因子: 3.4
作者:
Powers,L;Chance,B;Ching,Y;Angiolillo,P
通讯作者: Angiolillo,P
细胞色素c氧化酶中细胞色素a与细胞色素a3的比例
DOI: --
发表时间: 1964
期刊:
影响因子: --
作者:
B. F. Gelder;A. Muijsers
通讯作者: A. Muijsers
细胞色素氧化酶:合成
DOI: --
发表时间: 1981
期刊:
影响因子: --
作者:
M. Wikström;K. Krab;M. Saraste
通讯作者: M. Saraste