Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea
Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea
复制标题
DOI：
10.1016/j.soilbio.2005.02.025
复制
发表时间：
2005-10-01
影响因子：
9.7
通讯作者：
Sa, T
中科院分区：
文献类型：
--
作者：
Chung, H;Park, M;Sa, T
采用全细胞脂肪酸甲酯(FAME)图谱和16S rDNA序列分析方法，从韩国不同作物根际土壤中分离和鉴定了具有体外解磷活性的细菌群。在皮科夫斯卡亚的培养基上生长的数百个菌落中，根据不溶性磷酸盐在液体培养中的增溶作用，选出了13个最好的菌株，并进一步进行了鉴定和鉴定。3株代表菌株的序列已登录GenBank核苷酸序列数据库，登录号分别为AY335552、AY335553、AY335554。(C)2005。
Whole-cell fatty acids methyl ester (FAME) profile and 16S rDNA sequence analysis were employed to isolate and identify the bacterial groups that actively solubilized phosphates in vitro from rhizosphere soil of various crops of Korea. Out of several hundred colonies that grew on Pikovskaya's medium 13 best isolates were selected based on the solubilization of insoluble phosphates in liquid culture and further characterized and identified. They were clustered under the genera Enterobacter, Pantoea and Klebsiella and the sequences of three representative strains were deposited in the GenBank nucleotide sequence data library under the accession numbers AY335552, AY335553, AY335554.(c) 2005 Published by Elsevier Ltd.