Detection of pathogenic elephant endotheliotropic herpesvirus in routine trunk washes from healthy adult Asian elephants (Elephas maximus) by use of a real-time quantitative polymerase chain reaction assay
Detection of pathogenic elephant endotheliotropic herpesvirus in routine trunk washes from healthy adult Asian elephants (Elephas maximus) by use of a real-time quantitative polymerase chain reaction assay
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DOI：
10.2460/ajvr.71.8.925
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发表时间：
2010-08-01
影响因子：
1
通讯作者：
Ling, Paul D.
中科院分区：
文献类型：
--
作者：
Stanton, Jeffrey J.;Zong, Jian-Chao;Ling, Paul D.
为了研究大象嗜内皮疱疹病毒（Elephant endotheliotropic herpesvirus，EEHV 1）的致病机制和传播途径，采用一种新型的EEHV 1特异性实时荧光PCR方法，对来自同一象群的5头健康圈养亚洲象（Elephas maximus）的体液样品进行了分析。该测定用于评价在15周期间从5头大象获得的配对全血和躯干洗涤样品。对EEHV 1阳性结果的树干清洗DNA制备物进行脱氧核糖核酸测序和病毒基因亚型分析。病毒基因亚型进行了比较与过去的致命病例疱疹病毒相关的疾病在hered.Results-The PCR检测检测病毒DNA的水平为1,200拷贝/毫升的全血。它被用来检测EEHV 1在树干分泌物中的3个调查的5头大象在15周的时间。病毒基因分型分析确定了2个不同的大象疱疹病毒，其中1个是相同的病毒与以前的致命的疱疹病毒相关疾病的情况下，在hered.Conclusions和临床相关性EEHV 1脱落在健康的亚洲象的躯干分泌物。树干分泌物可能是这种病毒的传播方式。本研究的结果可能是有用的EEHV 1相关疾病的诊断，治疗和管理和圈养大象种群的整体管理。(Am J Vet Res 2010;71：925-933）
Objective-To investigate the pathogenesis and transmission of elephant endotheliotropic herpesvirus (EEHV1) by analyzing various elephant fluid samples with a novel EEHV1-specific real-time PCR assay.Animals-5 apparently healthy captive Asian elephants (Elephas maximus) from the same herd.Procedures-A real-time PCR assay was developed that specifically detects EEHV1. The assay was used to evaluate paired whole blood and trunk-wash samples obtained from the 5 elephants during a 15-week period. Deoxyribonucleic acid sequencing and viral gene subtyping analysis were performed on trunk-wash DNA preparations that had positive results for EEHV1. Viral gene subtypes were compared with those associated with past fatal cases of herpesvirus-associated disease within the herd.Results-The PCR assay detected viral DNA to a level of 1,200 copies/mL of whole blood. It was used to detect EEHV1 in trunk secretions of 3 of the 5 elephants surveyed during the 15-week period. Viral gene subtyping analysis identified 2 distinct elephant herpesviruses, 1 of which was identical to the virus associated with a previous fatal case of herpesvirus-associated disease within the herd.Conclusions and Clinical Relevance-EEHV1 was shed in the trunk secretions of healthy Asian elephants. Trunk secretions may provide a mode of transmission for this virus. Results of this study may be useful for the diagnosis, treatment, and management of EEHV1-associated disease and the overall management of captive elephant populations. (Am J Vet Res 2010;71:925-933)