Ammonium removal performance of anaerobic ammonium-oxidizing bacteria immobilized in polyethylene glycol gel carrier - Anammox bacteria immobilized in gel carrier
Ammonium removal performance of anaerobic ammonium-oxidizing bacteria immobilized in polyethylene glycol gel carrier - Anammox bacteria immobilized in gel carrier
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DOI：
10.1007/s00253-007-1106-6
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发表时间：
2007-10-01
影响因子：
5
通讯作者：
Tsuneda, Satoshi
中科院分区：
文献类型：
--
作者：
Isaka, Kazuichi;Date, Yasuhiro;Tsuneda, Satoshi
以聚乙二醇凝胶为载体固定化厌氧氨氧化菌。将少量的种子污泥[0.24%（w/v）]截留在载体中，并进行连续进料试验。脱氮活性逐渐增加，在第67天达到3.7 kg N/m（3）停止反应器每天。氮转化率的平均值计算为3.4kgN/m3反应器/天。显微镜检查清楚地显示在凝胶载体中形成小的红色簇。荧光原位杂交分析表明，这些簇由厌氧氨氧化细菌组成。从实时聚合酶链反应分析，厌氧氨氧化细菌在凝胶载体中的生长通过在第41天和第55天之间浮游菌的16 S rRNA基因的浓度从4.3 x 10（8）增加到4.2 x 10（9）拷贝/ml而清楚地显示。为了确定接种对反应器启动的影响，改变凝胶载体中的种子污泥的量，并且发现当使用1.4%（w/v）的污泥浓度时，启动期可以减少到25天。这是第一次成功地在凝胶载体中固定化和培养厌氧氨氧化细菌的报道。
Anaerobic ammonium-oxidizing (anammox) bacteria were immobilized in polyethylene glycol gel carriers. A small amount of seed sludge [0.24% (w/v)] was entrapped in the carriers, and continuous feeding tests were performed. Nitrogen removal activity increased gradually, reaching 3.7 kg N/m(3) stop reactor per day on day 67. The average of nitrogen conversion rate was calculated as 3.4 kg N/m(3) reactor per day. Microscopic examination clearly showed that small red clusters formed in the gel carrier. Moreover, fluorescence in situ hybridization analysis revealed that these clusters consisted of anammox bacteria. From real-time polymerase chain reaction analysis, the growth of anammox bacteria in the gel carriers was clearly shown by increased concentration of 16S rRNA gene of planctomycete from 4.3 x 10(8) to 4.2 x 10(9) copies/ml between days 41 and 55. To determine the effects of inoculation on the start-up of the reactor, the amount of seed sludge in the gel carrier was varied and it was found that the start-up period could be reduced to as little as 25 days when a sludge concentration of 1.4% (w/v) was used. This is the first report of successful immobilization and cultivation of anammox bacteria in a gel carrier.