Sexual transmission of human T-cell leukemia virus type I associated with the presence of anti-Tax antibody.
Sexual transmission of human T-cell leukemia virus type I associated with the presence of anti-Tax antibody.
复制标题
人类 T 细胞白血病病毒 I 型的性传播与抗 Tax 抗体的存在有关。
DOI：
10.1073/pnas.88.4.1182
复制
发表时间：
1991
影响因子：
11.1
通讯作者：
Essex,M
中科院分区：
文献类型：
--
作者：
Chen,YM;Okayama,A;Lee,TH;Tachibana,N;Mueller,N;Essex,M
人类T细胞白血病病毒I型（HTLV-I）的tax基因产物（Tax蛋白）是病毒长末端重复序列的特异性转录激活剂，对于病毒的复制周期至关重要。为了阐明抗 Tax 抗体的存在与病毒感染传播之间的关系，对来自 HTLV-I 血清学不一致或一致的已婚夫妇的年度连续血清样本进行了检查。其中包括 5 名个人，他们的配偶在 5 年的跟踪研究期间发生了血清转化。使用重组 Tax 蛋白作为抗原，通过蛋白质印迹测定对样品进行测试。结果显示，一致夫妇（丈夫和妻子均为HTLV-I携带者）中的32名男性中，有24名（75%）具有抗Tax抗体，而不一致夫妇（丈夫是携带者，妻子是HTLV-I血清阴性）中的18名男性中，只有5名（27.8%）抗Tax抗体呈阳性（P = 0.0012）。此外，5 名血清转化者（4 名女性和 1 名男性）的所有配偶均具有抗 Tax 抗体，而来自不一致夫妇组的 46 名年龄匹配的随机选择的 HTLV-I 携带者中，只有 23 名 (50%) 具有抗 Tax 抗体。按性别对数据进行分析时，女性血清转化者的所有丈夫均具有抗Tax抗体，显着高于随访期间未将病毒传播给配偶的男性抗Tax抗体患病率（P = 0.017）。此外，还检查了对其他HTLV-I抗原（包括Env gp46、跨膜蛋白gp21以及Gag p19和p24）的抗体反应性。结果表明，血清转化者和参照组的配偶中对任何抗原的抗体反应性流行率没有显着差异。我们的结论是，男性体内存在抗泰克斯抗体可能表明病毒通过异性途径传播给妻子的风险很高。
The tax gene product (Tax protein) of human T-cell leukemia virus type I (HTLV-I) is a specific transcriptional activator of the viral long terminal repeat sequence and is essential for the replication cycle of the virus. To elucidate the relationship between the presence of anti-Tax antibody and the transmission of the viral infection, annual consecutive serum samples from married couples serologically discordant or concordant for HTLV-I were examined. These included 5 individuals whose spouses seroconverted during this 5-year follow-up study period. The samples were tested by a Western blot assay using a recombinant Tax protein as the antigen. The results showed that 24 of 32 (75%) men in the concordant couples (both husband and wife were HTLV-I carriers) had anti-Tax antibody, while only 5 of 18 (27.8%) men in the discordant couples (husband was carrier and wife was seronegative to HTLV-I) were positive for anti-Tax antibody (P = 0.0012). Furthermore, all spouses of the 5 seroconverters (4 women and 1 man) had anti-Tax antibody, while only 23 of 46 (50%) age-matched randomly selected HTLV-I carriers from the discordant-couple group had anti-Tax antibody. When the data were analyzed by gender, all husbands of the female seroconverters had anti-Tax antibodies, which was significantly higher than the prevalence of anti-Tax antibodies in men who did not transmit the virus to their spouses during the follow-up period (P = 0.017). In addition, antibody reactivity to other HTLV-I antigens (including Env gp46, transmembrane protein gp21, and Gag p19 and p24) were examined. The results indicated no significant differences between the prevalence of antibody reactivity to any of the antigens in the spouses of the seroconverters and the reference group. We conclude that the presence of anti-Tax antibody in men may indicate a high risk of viral transmission to their wives via heterosexual routes.