SEPARATION OF HERPES-SIMPLEX VIRUS VIRIONS AND NUCLEOCAPSIDS ON PERCOLL GRADIENTS
SEPARATION OF HERPES-SIMPLEX VIRUS VIRIONS AND NUCLEOCAPSIDS ON PERCOLL GRADIENTS
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DOI：
10.1016/0166-0934(80)90047-6
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
LYCKE, E
中科院分区：
文献类型：
--
作者：
SVENNERHOLM, B;VAHLNE, A;LYCKE, E
采用聚乙烯吡咯烷酮包被二氧化硅（Percoll）密度梯度离心分离法分离纯化单纯疱疹病毒（HSV）病毒粒子和核衣壳。病毒粒子和核衣壳的密度分别为1.07和1.03 g/ml。病毒粒子和核衣壳在梯度上的分布表明，粒子是根据大小而不是密度来区分的。纯化病毒粒子制备过程中细胞蛋白的还原量为原来的1300 ~ 2100倍。感染病毒的回收率约为0.1%。30%。
Herpes simplex virus (HSV) virions and nucleocapsids were separated and purified by centrifugation in density gradient of polyvinylpyrrolidone-coated silica (Percoll). Virions and nucleocapsids banded at densities of 1.07 and 1.03 g/ml, respectively. The distribution in the gradient of virions and nucleocapsids suggested that particles were discriminated according to difference in size rather than in density. The reduction of cell proteins in preparations of purified virions was 1300-2100 times. The recovery of infective virus was .apprx. 30%.