Structural analysis of the polysaccharide from the lipopolysaccharide of Porphyromonas gingivalis strain W50
Structural analysis of the polysaccharide from the lipopolysaccharide of Porphyromonas gingivalis strain W50
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DOI：
10.1046/j.1432-1327.2001.02397.x
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发表时间：
2001-09-01
期刊：
影响因子：
--
通讯作者：
Hounsell, EF
中科院分区：
文献类型：
--
作者：
Paramonov, N;Bailey, D;Hounsell, EF
牙龈卟啉单胞菌的脂多糖是牙周病中重要的促炎分子，也是宿主特异性免疫反应的重要靶点。此外，我们最近使用单克隆抗体证明了牙龈假单胞菌的精氨酸牙龈痛是由免疫相关的糖链翻译后修饰的--这与这种微生物的一种内毒素制剂有关。在本研究中，我们根据一维和二维核磁共振(DQF-COSY、TOCSY、NOESY、ROESY、H-1-C-13HSQC和H-1-P-31HXTOCSY)和GC-MS数据确定了牙龈假单胞菌W50的O-多糖的结构。这些数据使我们得出结论，O-多糖是由四糖重复序列组成的：-6)-α-D-Glcp-(1-->；4)-α-L-Rhap-(1-->；3)-β-D-GalNAc-(1-->；3)-α-D-Galp-(1-->；)-α-D-Galp-(1-->；)，并在α-鼠李糖残基的C-2位携带非化学计量比(约60%)的单磷乙醇胺残基。这些数据表明牙龈假单胞菌的O-多糖是由一个异常修饰的四糖重复单位组成的。
The lipopolysaccharide (LPS) of Porphyromonas gingivalis is an important pro-inflammatory molecule in periodontal disease and a significant target of the host's specific immune response. In addition, we recently demonstrated using monoclonal antibodies that the Arg-gingipains of P. gingivalis are post-translationally modified with glycan chains that are immunologically related - to an LPS preparation from this organism. In the present investigation, we determined the structure of the O-polysaccharide of P. gingivalis W50 that was fully characterized on the basis of 1D and 2D NMR (DQF-COSY, TOCSY, NOESY, ROESY, H-1-C-13 HSQC and H-1-P-31 HXTOCSY) and GC-MS data. These data allowed us to conclude that the O-polysaccharide is built up of the tetrasaccharide repeating sequence: -->6)-alpha -D-Glcp-(1-->4)-alpha -L-Rhap-(1-->3)-beta -D-GalNAc-(1-->3)-alpha -D-Galp-(1--> and carries a monophosphoethanolamine residue at position C-2 of the alpha -rhamnose residue in a nonstoichiometric (approximately 60%) amount. These data indicate that the O-polysaccharide of P. gingivalis LPS is composed of an unusually modified tetrasaccharide repeating unit.