Photocatalytically patterned TiO2 arrays for on-plate selective enrichment of phosphopeptides and direct MALDI MS analysis.
Photocatalytically patterned TiO2 arrays for on-plate selective enrichment of phosphopeptides and direct MALDI MS analysis.
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DOI：
10.1021/ac1024232
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发表时间：
2011-03-01
影响因子：
7.4
通讯作者：
Cheng Q
中科院分区：
文献类型：
--
作者：
Wang H;Duan J;Cheng Q
我们报告的光催化图案化的二氧化钛阵列的选择性板上富集和直接基质辅助激光解吸/电离质谱（MALDI-MS）分析的磷酸肽的发展。通过逐层（LbL）沉积/煅烧工艺在金覆盖的载玻片上制备具有受控孔隙率的薄TiO 2纳米膜。高度多孔且粗糙的纳米结构为磷酸化物质的选择性结合提供了高表面积。通过使用十八烷基三氯硅烷（OTS）涂层结合UV照射与光掩模，随后NaOH蚀刻来产生图案化阵列。由此产生的亲水性TiO 2点被疏水性OTS层包围，这可以通过将大体积样品限制在小区域内来促进低丰度组分的富集。二氧化钛阵列表现出高特异性对磷酸肽在复杂的样品，包括磷蛋白酶和人血清，和检测可以在fmole范围内。阵列的其他优点包括优异的稳定性、可重复使用性/再现性和低成本。该方法已成功地应用于脱脂乳中磷酸肽的分析。图案化的二氧化钛阵列提供了一个有吸引力的界面，用于进行板上反应，包括选择性捕获MALDI-MS分析的目标物种，并可以作为一个通用的实验室芯片平台，用于磷酸化蛋白质组研究的高通量分析。
We report the development of photocatalytically patterned TiO2 arrays for selective on-plate enrichment and direct matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis of phosphopeptides. A thin TiO2 nanofilm with controlled porosity is prepared on gold-covered glass slides by a layer-by-layer (LbL) deposition/calcination process. The highly porous and rough nanostructure offers high surface area for selective binding of phosphorylated species. The patterned arrays are generated by using an octadecyltrichlorosilane (OTS) coating in combination of UV irradiation with a photomask, followed by NaOH etching. The resulting hydrophilic TiO2 spots are thus surrounded by a hydrophobic OTS layer, which can facilitate the enrichment of low-abundance components by confining a large volume sample into a small area. The TiO2 arrays exhibit high specificity towards phosphopeptides in complex samples including phosphoprotein digests and human serum, and the detection can be made in the fmole range. Additional advantages of the arrays include excellent stability, reusability/reproducibility, and low cost. This method has been successfully applied to the analysis of phosphopeptides in non-fat milk. The patterned TiO2 arrays provide an attractive interface for performing on-plate reactions including selective capture of target species for MALDI-MS analysis, and can serve as a versatile lab-on-a-chip platform for high throughput analysis in phosphoproteome research.
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影响因子：
4
作者：
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通讯作者：
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影响因子：
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