Antioxidant, antibacterial, and cytotoxic activities of Cedrus atlantica organic extracts and essential oil
Antioxidant, antibacterial, and cytotoxic activities of Cedrus atlantica organic extracts and essential oil
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DOI：
10.1016/j.eujim.2021.101292
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发表时间：
2021-02-03
影响因子：
2.5
通讯作者：
Amrine, Chiraz Soumia M.
中科院分区：
文献类型：
--
作者：
Belkacem, Nassim;Khettal, Bachra;Amrine, Chiraz Soumia M.
简介：大西洋杉木是一种特有的松树，以其木材油而闻名。其传统药用用途主要是消炎和抗菌。目前的研究旨在从化学和生物学上探索这种树的球果精油和树枝的有机提取物。方法：采用气相色谱-质谱联用技术（GC-MS）对挥发油进行分析。根据文献中描述的不同方法测定提取物和馏分的酚含量。以抗氧化（FRAP、DPPH中心点和ABTS（中心点+）自由基清除活性）、抗菌（微量肉汤稀释法）和细胞毒性（MTT法）进行生物活性评价。结果：挥发油的主要成分为α -蒎烯（81.49%）。提取物和馏分中含有丰富的多酚。乙酸乙酯部分的抗氧化活性较好。金黄色葡萄球菌对大西洋青霉提取物和挥发油最敏感，乙酸乙酯部分的最低抑菌浓度和最低杀菌浓度分别为62.5和125 μ g/mL，挥发油的最低抑菌浓度分别为0.25和0.5%。对MCF-7乳腺癌细胞具有较强的杀伤活性，IC50值为143.13 +/- 14.6 μ g/mL。结论：大西洋花挥发油及其有机提取物具有抗氧化、抗菌和抗癌活性。因此，在传统制剂中的民族植物学用途值得研究，因为该植物似乎是有趣的代谢物的潜在来源。
Introduction: Cedrus atlantica is an endemic pine tree species well known for its wood oil. Its traditional medicinal usages are mainly anti-inflammatory and antibacterial. The current study aimed to explore both the essential oil of the tree's cones and the organic extracts of the branches, chemically and biologically.Methods: The essential oil was analyzed using gas chromatography coupled with mass spectrometry (GC-MS). Phenolic contents of the extracts and fractions were determined following diverse methods described in the literature. The bioactivity was assessed for their antioxidant (FRAP, DPPH center dot and ABTS(center dot+) radical scavenging activities), antibacterial (Broth microdilution method), and cytotoxic effects (MTT assay).Results: alpha-pinene (81.49%) was found to be the major constituent of the essential oil. The extracts and fractions were found to be rich in polyphenols. The antioxidant activity was better in the ethyl acetate fraction. Staphylococcus aureus appeared to be the most susceptible strain to C. atlantica's extracts and oil, with a minimum inhibitory concentration and minimum bactericidal concentration values of 62.5 and 125 mu g/mL for the ethyl acetate fraction, respectively, and values of 0.25 and 0.5% for the essential oil, respectively. C. atlantica's essential oil exhibited a potent cytotoxic activity against MCF-7 breast cancer cell line with an IC50 value of 143.13 +/- 14.6 mu g/mL.Conclusion: C. atlantica's essential oil and organic extracts showed antioxidant, antibacterial, and anticancer activities. Thereby, the ethnobotanical use of C. atlantica in traditional preparations is worth investigating as the plant appears to be a potential source of interesting metabolites.