tdrd1 is a germline-specific and sexually dimorphically expressed gene in Paralichthys olivaceus
tdrd1 is a germline-specific and sexually dimorphically expressed gene in Paralichthys olivaceus
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tdrd1 是牙鲆种系特异性和性二态性表达的基因
DOI：
10.1016/j.gene.2018.06.043
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Zhang Quanqi
中科院分区：
文献类型：
--
作者：
Zhao Jun;Wang Bo;Yu Haiyang;Wang Yujue;Liu Xiaobing;Zhang Quanqi
Tudor结构域蛋白1（tdrd 1）是Tudor家族的成员，在胚胎发生和配子发生过程中发挥重要作用。本研究克隆了比目鱼（Paralichthys olivaceustdrd 1）的全长cDNA（Potdrd 1）。PoTDRD 1是一种多结构域蛋白，具有N-末端MYND锌指结构域，随后是四个串联延伸的Tudor结构域。序列比较、基因组结构分析、系统发育分析和同线性分析表明，Potdrd 1与其它硬骨鱼类的Potdrd 1基因同源性较高。原位杂交（ISH）结果表明，Potdrd 1 mRNA在卵巢卵原细胞和卵母细胞以及精巢精原细胞和精母细胞中均有表达。在幼鱼性腺分化过程中，Potdrd 1 mRNA的表达量从30 dph到100 dph迅速增加，并表现出明显的性二态性，与抗苗勒管激素（amh）的表达一致; Potdrd 1 mRNA在胚胎发生过程中始终存在，从未受精卵到囊胚期表达量较高，随后逐渐下降，直至孵化。将含有Potdrd 1基因3′非翻译区（UTR）和绿色荧光蛋白（GFP）的嵌合RNA注射到斑马鱼受精卵中，仅在推测的PGCs中观察到绿色荧光。这些结果表明，Potdrd 1是一个生殖系特异性和性二态性因子，可能在生殖系发育和配子发生中发挥作用。
Tudor domain containing protein 1 (tdrd1) is a member of the Tudor family and has shown essential functions during embryogenesis and gametogenesis. In this study, we cloned the full length cDNA ofParalichthys olivaceustdrd1(Potdrd1). PoTDRD1 is a multidomain protein with an N-terminal MYND zinc finger domain, followed by four tandem extended Tudor domains. Sequence comparison, genomic structure, phylogenetic analyses and synteny analyses showed thatPotdrd1was homologous to those of other teleosts. In adult individuals, the expression ofPotdrd1was higher in testis than in ovary, demonstrating a sexually dimorphic gene expression pattern.In situhybridization (ISH) showed thatPotdrd1mRNA was detected in oogonia and oocytes of ovary as well as in spermatogonia and spermatocytes of testis. In juveniles during gonad differentiation its expression level increased rapidly from 30 dph to 100 dph and showed obvious sexual dimorphism that was in accordance with the expression of anti-Mullerian hormone (amh).Potdrd1mRNA was consistently detected during embryogenesis, and its level was higher from unfertilzed eggs to the blastula stage and subsequently decreased until hatching. When chimeric RNA containing green fluorescent protein (GFP) and 3′ untranslated regions (UTR) ofPotdrd1was microinjected into zebrafish fertilized eggs, the green fluorescence could be visualized only in putative PGCs. These results indicated thatPotdrd1is a germline specific and sexually dimorphic factor that potentially functionate in germline development and gametogenesis in Japanese flounder.