Optimizing Docosahexaenoic Acid (DHA) Production by Schizochytrium sp. Grown on Waste Glycerol
Optimizing Docosahexaenoic Acid (DHA) Production by Schizochytrium sp. Grown on Waste Glycerol
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DOI：
10.3390/en14061685
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发表时间：
2021-03-01
期刊：
影响因子：
3.2
通讯作者：
Zielinski, Marcin
中科院分区：
文献类型：
--
作者：
Kujawska, Natalia;Talbierz, Szymon;Zielinski, Marcin
本研究的目的是优化以废甘油为有机碳源生长的裂殖壶菌的生物量和二十二碳六烯酸（DHA）产量。使用部分Plackett-Burman设计和响应面法（RSM）筛选对生物量和DHA产量具有显著影响的参数。生长培养基中的粗甘油浓度（150 g/dm（3））、工艺温度（27 ℃）、生物反应器中的氧气（49.99% v/v）和作为氮源的蛋白胨浓度（9.99 g/dm（3））对裂殖壶菌生长的影响最为显著。确定为在生物质中产生高DHA浓度的最佳工艺参数值如下：甘油浓度149.99 g/dm（3），温度26 ℃，氧浓度30%（v/v），和蛋白胨浓度2.21 g/dm（3）。在实际实验室条件下获得的干细胞重量（DCW）为66.69 +/- 0.66 g/dm（3），即，比预测值低1.27%。实际培养中获得的DHA浓度为17.25 +/- 0.33 g/dm（3），比预测值低3.03%。所获得的结果表明，应采用两步培养系统，第一阶段集中于高产量的裂殖壶菌属生物量，第二阶段集中于增加细胞中的DHA浓度。
The aim of this study was to optimize biomass and docosahexaenoic acid (DHA) production by Schizochytrium sp. grown on waste glycerol as an organic carbon source. Parameters having a significant effect on biomass and DHA yields were screened using the fractional Plackett-Burman design and the response surface methodology (RSM). Schizochytrium sp. growth was most significantly influenced by crude glycerin concentration in the growth medium (150 g/dm(3)), process temperature (27 degrees C), oxygen in the bioreactor (49.99% v/v), and the concentration of peptone as a source of nitrogen (9.99 g/dm(3)). The process parameter values identified as optimal for producing high DHA concentrations in the biomass were as follows: glycerin concentration 149.99 g/dm(3), temperature 26 degrees C, oxygen concentration 30% (v/v), and peptone concentration 2.21 g/dm(3). The dry cell weight (DCW) obtained under actual laboratory conditions was 66.69 +/- 0.66 g/dm(3), i.e., 1.27% lower than the predicted value. The DHA concentration obtained in the actual culture was at 17.25 +/- 0.33 g/dm(3), which was 3.03% lower than the predicted value. The results obtained suggest that a two-step culture system should be employed, with the first phase focused on high production of Schizochytrium sp. biomass, and the second focused on increasing DHA concentration in the cells.