Detection of serum antibodies to retroviral proteins in patients with primary Sjögren's syndrome (autoimmune exocrinopathy).
Detection of serum antibodies to retroviral proteins in patients with primary Sjögren's syndrome (autoimmune exocrinopathy).
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原发性干燥综合征（自身免疫性外分泌病）患者血清中逆转录病毒蛋白抗体的检测。
DOI：
10.1002/art.1780330603
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发表时间：
1990
影响因子：
--
通讯作者：
Garry,RF
中科院分区：
文献类型：
--
作者：
Talal,N;Dauphinée,MJ;Dang,H;Alexander,SS;Hart,DJ;Garry,RF
原发性Sjögren综合征（SS）被认为是一种良性自身免疫性疾病；其特征是唾液腺和泪腺淋巴浸润，常伴有血清自身抗体的存在，特别是抗ro （SS-A）和抗la （SS-B）。原发性SS和获得性免疫缺陷综合征之间有重要的免疫学相似性。为了研究原发性SS对逆转录病毒蛋白可能的免疫应答，我们对47例原发性SS患者的血清进行了针对人类免疫缺陷病毒-1 （HIV-1）蛋白的免疫印迹检测。在14例（30%）患者中发现了中强反应性，表明血清抗体的存在。在120名正常受试者中，只有1人表现为中度阳性。所有14个阳性SS血清对p24 （gag）均有反应，但对gp41或gp120 （env）均无反应。这种反应并不反映高γ球蛋白血症，因为29例SS患者的血清中含有抗呕吐反应性和不含有抗呕吐反应性的血清中的免疫球蛋白浓度是相同的。两种血清也对p17 gag有反应。其中四种与HIV-2核心蛋白发生反应，但没有一种与人T淋巴细胞嗜性病毒i的核心蛋白发生反应。14种血清中只有1种与Ro （SS-A）反应，另外1种与La （SS-B）反应。这些结果确定了SS患者的一个子集，其特征是1)存在HIV-1群体特异性的血清抗体，但不是类型特异性的蛋白质，以及2)相对缺乏抗ro （SS- a）和抗la （SS- b）自身抗体。在后一个方面，这些SS患者构成了一个亚群，类似于hiv诱导的SS样疾病患者。
Primary Sjögren's syndrome (SS) is considered a benign autoimmune disease; it is characterized by lymphoid infiltration of salivary and lacrimal glands, often accompanied by the presence of serum autoantibodies, particularly anti-Ro (SS-A) and anti-La (SS-B). There are important immunologic similarities between primary SS and acquired immunodeficiency syndrome. To investigate for a possible immune response to retroviral proteins in primary SS, we performed immunoblotting against human immunodeficiency virus-1 (HIV-1) proteins using sera from 47 patients with primary SS. Moderate-to-strong reactivity, suggesting the presence of serum antibodies, was found in 14 patients (30%). Of 120 normal subjects, only 1 showed moderate positivity. All 14 positive SS sera reacted against p24 (gag) but failed to react against gp41 or gp120 (env). This response did not reflect hypergammaglobulinemia since immunoglobulin concentrations among the 29 SS patients studied were the same in sera that contained and sera that did not contain anti-gag reactivity. Two sera also reacted against p17 gag. Four reacted against HIV-2 core proteins, but none reacted with core proteins of human T lymphotropic virus-I. Only 1 of the 14 sera reacted against Ro (SS-A), and 1 other reacted against La (SS-B). These results identify a subset of SS patients characterized by 1) the presence of serum antibodies to HIV-1 group-specific, but not type-specific, proteins, and 2) the relative absence of anti-Ro (SS-A) and anti-La (SS-B) autoantibodies. In this latter respect, these SS patients constitute a subpopulation that resembles patients with HIV-induced SS-like disease.