Clinical significance of miR-22 expression in patients with colorectal cancer
Clinical significance of miR-22 expression in patients with colorectal cancer
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DOI：
10.1007/s12032-012-0233-9
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发表时间：
2012-12-01
期刊：
影响因子：
3.4
通讯作者：
Zhou, Tong
中科院分区：
文献类型：
--
作者：
Zhang, Guangjun;Xia, Shusen;Zhou, Tong
微小RNA（miRNAs）是非编码RNA，在多种肿瘤中表达异常。在这些miRNAs中，miR-22作为肿瘤抑制因子已被证明在人类肿瘤发生中起关键作用。然而，其与结直肠癌的临床病理特征的关系尚未得到解决。本研究采用实时荧光定量RT-PCR方法，比较了miR-22在结直肠癌组织和癌旁正常黏膜中的表达。通过适当的统计学分析分析miR-22表达与临床病理特征的关联。采用Kaplan-Meier分析和考克斯比例风险回归模型分析miR-22表达与患者生存期的关系。结果显示，miR-22在大肠癌组织中的相对表达水平明显低于癌旁正常黏膜，且miR-22的低表达与肝转移相关。Kaplan-Meier分析表明，miR-22降低的患者总体生存率较差。此外，多变量分析显示，miR-22表达降低是总生存率的独立预测因子。我们的数据表明miR-22作为CRC新的预后生物标志物的潜力。
MicroRNAs (miRNAs) are non-coding RNAs that have been shown to be aberrantly expressed in several tumor types. Of these miRNAs, miR-22 as tumor suppressor has been shown to play a crucial role in human carcinogenesis. However, its association with the clinicopathological features of colorectal cancer has yet to be addressed. In this study, we compared the expression of miR-22 between colorectal cancer tissues and the normal adjacent mucosa using a quantitative real-time RT-PCR. The association of miR-22 expression with clinicopathological characters was analyzed by appropriate statistical analysis. Kaplan-Meier analysis and Cox proportional hazards regression models were used to investigate the associations of miR-22 expression with survival of patients. Results showed that the relative expression levels of miR-22 were significantly lower in colorectal cancer tissues than those in the normal adjacent mucosa, and low expression of miR-22 correlated with liver metastasis. Kaplan-Meier analysis indicated that patients with reduced miR-22 had a poor overall survival. Moreover, the multivariate analysis showed that reduced expression of miR-22 was an independent predictor of overall survival. Our data indicate the potential of miR-22 as a novel prognostic biomarker for CRC.