ELECTROPHORESIS OF RIBONUCLEIC-ACID ON A POLYACRYLAMIDE-GEL WHICH CONTAINS DISULFIDE CROSS-LINKAGES
ELECTROPHORESIS OF RIBONUCLEIC-ACID ON A POLYACRYLAMIDE-GEL WHICH CONTAINS DISULFIDE CROSS-LINKAGES
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DOI：
10.1016/0003-2697(76)90261-x
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发表时间：
1976-01-01
影响因子：
2.9
通讯作者：
HANSEN, JN
中科院分区：
文献类型：
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作者：
HANSEN, JN
合成了一种含二硫化物的双丙烯酰胺类似物。当在聚丙烯酰胺-凝胶形成中取代双丙烯酰胺作为交联剂时，形成的凝胶具有与普通凝胶相当相似的电泳特性。由二硫交联形成的凝胶在不降解RNA的条件下可溶于2-巯基乙醇。通过电泳分离的蜡样芽孢杆菌的RNA样品通过离子交换色谱法从溶解的凝胶中回收，总收率约为65%。还原后的聚合物可以通过大气氧化重新凝胶化。二硫交联提供了凝胶基质的多功能性和物理状态的控制，这是以前没有的。
A disulfide-containing analog of bis-acrylamide was synthesized. When substituted for bis-acrylamide as the cross-linking agent in polyacrylamide-gel formation, a gel is formed which has electrophoretic properties that are quite similar to normal gels. Gels formed with the disulfide cross-link are soluble in the presence of 2-mercaptoethanol under conditions which do not degrade RNA. RNA samples [from Bacillus cereus] which had been separated electrophoretically were recovered in about 65% overall yield from the dissolved gel by ion-exchange chromatography. The reduced polymer can be re-gelled by atmospheric oxidation. Disulfide cross-links provide versatility and control of the physical state of the gel matrix which were not previously available.