Carbon nanotube DNA sensor and sensing mechanism
Carbon nanotube DNA sensor and sensing mechanism
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DOI：
10.1021/nl060613v
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发表时间：
2006-08-09
期刊：
影响因子：
10.8
通讯作者：
Wang, Qian
中科院分区：
文献类型：
--
作者：
Tang, Xiaowu;Bansaruntip, Sarunya;Wang, Qian
本文报道了单壁碳纳米管（SWNT） DNA传感器的制备及其传感机理。简单和通用的协议，证明了DNA杂交无标记检测随机序列15mer和30mer寡核苷酸。DNA杂交在金电极上，而不是在SWNT侧壁上，主要是由于SWNT和金接触之间的能级排列调制而导致的电导率的急剧变化。这项工作为SWNT-DNA结合对DNA功能的影响提供了具体的实验证据，这将有助于为未来设计用于生物技术的SWNT生物复合物以及DNA辅助纳米管操作技术铺平道路。
We report the fabrication of single-walled carbon nanotube (SWNT) DNA sensors and the sensing mechanism. The simple and generic protocol for label-free detection of DNA hybridization is demonstrated with random sequence 15mer and 30mer oligonucleotides. DNA hybridization on gold electrodes, instead of on SWNT sidewalls, is mainly responsible for the acute electrical conductance change due to the modulation of energy level alignment between SWNT and gold contact. This work provides concrete experimental evidence on the effect of SWNT-DNA binding on DNA functionality, which will help to pave the way for future designing of SWNT biocomplexes for applications in biotechnology in general and also DNA-assisted nanotube manipulation techniques.