Enhancement of polysaccharide production by optimization of culture conditions in shake flask submerged cultivation of Grifola umbellata
Enhancement of polysaccharide production by optimization of culture conditions in shake flask submerged cultivation of Grifola umbellata
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DOI：
10.1016/j.jcice.2008.01.003
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发表时间：
2008-07-01
期刊：
影响因子：
--
通讯作者：
Liu, Yung-Chuan
中科院分区：
文献类型：
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作者：
Huang, Hung-Chang;Liu, Yung-Chuan
猪苓是一种传统的中药用食用菌。本文研究了培养条件对菌丝生长和胞外多糖(EPS)产量的影响。葡萄糖是菌丝生长和产胞外多糖的最佳碳源。酵母膏是菌丝生长的最佳氮源，脱脂乳作为氮源能显著提高EPS产量。产胞外多糖的最佳培养基组成为：葡萄糖3%，脱脂乳0.2%，KH(2)PO(4)0.1%，MgSO(4)0~1%，7H(2)O，维生素B1 0.005%。初始pH值为5对EPS的产生效果最好。以脱脂乳为氮源的猪栖菜培养菌胞外多糖比产量高达112.35 mg/g，是复合氮源培养的4.36倍。(C)2008年台湾化学工程师学会。爱思唯尔出版，版权所有。
Grifola umbellata (Fries) is a traditional Chinese medicinal mushroom. In this paper, the effects of cultural condition on the mycelial growth and exopolysaccharide (EPS) production were studied. Glucose was the best carbon source for mycelia growth and EPS production. Yeast extract was the best nitrogen source for mycelia growth, whereas skim milk as nitrogen source can remarkably improve EPS production. The optimal medium constituents for EPS production were as follows: glucose 3%, skim milk 0.2%, 0.1% KH(2)PO(4), 0-1% MgSO(4), 7H(2)O and 0.005% vitamin B1. The initial pH value of 5 was the most efficient to EPS production. The G. umbellata culture with skim milk as nitrogen source displayed a much higher specific EPS yield of 112.35 mg/g, accounting for a 4.36 times increase compared to that with combined nitrogen source medium. (c) 2008 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.