Cct1, a phosphatidylcholine biosynthesis enzyme, is required for Drosophila oogenesis and ovarian morphogenesis
Cct1, a phosphatidylcholine biosynthesis enzyme, is required for Drosophila oogenesis and ovarian morphogenesis
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DOI：
10.1242/dev.00817
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发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Schüpbach, T
中科院分区：
文献类型：
--
作者：
Gupta, T;Schüpbach, T
果蝇卵的形成需要生殖细胞和周围的体细胞卵泡细胞之间的合作。为了确定参与卵泡细胞模式的基因，我们分析了在卵泡细胞的特定亚群中表达的增强子陷阱系。通过这一分析，我们确定了与CTP同源的串联果蝇基因：磷酸胆碱胞基转移酶（CCT），这是cdp -胆碱途径中三种酶中的第二种，用于合成磷脂酰胆碱。果蝇Cct1在三个特定的卵泡细胞亚群中高水平表达，这种表达至少部分受到tgf - β和Egfr信号通路的调节。Cct1的突变导致许多缺陷，包括生殖系干细胞维持能力丧失、卵母细胞定位错误和卵盖缩短，这表明Cct1在卵子发生过程中发挥了多种作用。此外，Cct1突变体显示出一种新的卵巢分支表型，表明在卵巢形态发生过程中需要该基因。这些数据首次证明了CCT和磷脂酰胆碱在发育过程中的模式形成中具有特定作用。
Patterning of the Drosophila egg requires cooperation between the germline cells and surrounding somatic follicle cells. In order to identify genes involved in follicle cell patterning, we analyzed enhancer trap lines expressed in specific subsets of follicle cells. Through this analysis, we have identified tandem Drosophila genes homologous to CTP: phosphocholine cytidylyltransferase (CCT), the second of three enzymes in the CDP-choline pathway, which is used to synthesize phosphatidylcholine. Drosophila Cct1 is expressed at high levels in three specific subsets of follicle cells, and this expression is regulated, at least in part, by the TGF-beta and Egfr signaling pathways. Mutations in Cct1 result in a number of defects, including a loss of germline stem cell maintenance, mispositioning of the oocyte, and a shortened operculum, suggesting that Cct1 plays multiple roles during oogenesis. In addition, Cct1 mutants display a novel branched ovariole phenotype, demonstrating a requirement for this gene during ovarian morphogenesis. These data provide the first evidence for a specific role for CCT, and thus for phosphatidylcholine, in patterning during development.