Localization of Simian immunodeficiency virus-infected cells in the genital tract of male and female Rhesus macaques
Localization of Simian immunodeficiency virus-infected cells in the genital tract of male and female Rhesus macaques
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DOI：
10.1016/s0165-0378(98)00069-2
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发表时间：
1998-12-01
影响因子：
3.4
通讯作者：
Miller, CJ
中科院分区：
文献类型：
--
作者：
Miller, CJ
SIV/恒河猴HIV传播模型使人们对HIV性传播过程中病毒与宿主之间的相互作用有了更多的了解。SIV可以通过恒河猴阴茎包皮和龟头的完整粘膜（复层鳞状上皮）传播。SIV感染的细胞可以在雄性恒河猴生殖道的各个层面发现，并且SIV可以感染阴茎包皮粘膜上皮细胞。SIV可以通过软塑料小儿鼻饲管将无细胞病毒悬液注入阴道腔而传播给雌性恒河猴。在月经周期的任何特定时间点接种疫苗与动物对感染的易感性之间似乎没有任何相关性。此外，手术切除宫颈和子宫并不影响SIV生殖道传播所需的无细胞病毒剂量。因此，SIV的靶细胞存在于阴道粘膜中。在慢性感染的雌性恒河猴中，SIV感染的细胞存在于子宫、子宫颈和阴道中。SIV感染的CD 1a（+）和p55（+）朗格汉斯细胞也见于阴道的复层鳞状上皮。总之，这些结果与以下假设一致：病毒最初感染阴道中的抗原呈递细胞（巨噬细胞和朗格汉斯细胞），然后在引流淋巴结中进行后续几轮复制，然后扩散到更近端的淋巴结，最后扩散到血流和远端淋巴组织。(C)1998由Elsevier Science爱尔兰有限公司出版。保留所有权利。
The SIV/Rhesus macaque model of HIV transmission has led to an increased understanding of the interactions between virus and host during the sexual transmission of HIV. SIV can be transmitted across the intact mucosa (stratified squamous epithelium) of the foreskin and glans of the penis of Rhesus macaques. SIV-infected cells can be found at all levels of the male Rhesus macaque reproductive tract and SIV can infect cells in the mucosal epithelium of the foreskin of the penis. SIV can be transmitted to female Rhesus macaques by infusing a cell-free virus suspension into the vaginal canal through a soft plastic pediatric nasogastric feeding tube. There does not appear to be any correlation between inoculation at any particular point in the menstrual cycle and the susceptibility of an animal to infection. Furthermore, the surgical removal of the cervix and uterus did not affect the dose of cell-free virus required for the genital transmission of SIV. Thus, target cells for SIV are present in the vaginal mucosa. In chronically-infected female Rhesus macaques, SIV-infected cells are present in the uterus, cervix and vagina. SIV-infected CD1a(+) and p55(+) Langerhans cells are also found within the stratified squamous epithelium of the vagina. Taken together these results are consistent with the hypothesis that the virus initially infects antigen-presenting cells in the vagina (macrophages and Langerhans cells) and then subsequent rounds of replication occur in the draining lymph nodes prior to spreading to more proximal lymphoid nodes and finally to the bloodstream and distant lymphoid tissue. (C) 1998 Published by Elsevier Science Ireland Ltd. All rights reserved.