Optical Coherence Tomography of Human Kidney
Optical Coherence Tomography of Human Kidney
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DOI：
10.1016/j.juro.2009.12.091
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发表时间：
2010-05-01
影响因子：
6.6
通讯作者：
Chen, Yu
中科院分区：
文献类型：
--
作者：
Onozato, Maristela L.;Andrews, Peter M.;Chen, Yu
目的：实时、非侵入性地检测活体人肾的组织病理学状态，将是肾脏疾病诊断的一个重要进展。最近，我们报道了光学相干层析成像具有所需的高空间分辨率，能够以微米级的分辨率无创性地确定啮齿动物肾脏的组织病理学变化。建立了光学相干层析成像技术能否1)有效地穿透人肾周围结缔组织包膜，2)全面观察人肾表面，3)确定人肾微结构的组织病理学变化。材料和方法：使用配备扫频激光光源(1.3微米波长)的高速光学相干层析成像系统，获得4个离体人肾脏的断层图像。结果：光学相干断层扫描能够显示肾小管、肾小球和皮质血管，穿透深度可达2 mm，空间分辨率可达10针。我们在几分钟内以一种非侵入性的方式对整个人类肾脏进行了检查和成像。获得的光学相干断层扫描图像记录了肾小管、肾小球和间质的组织病理学变化，这与传统的组织学观察结果非常吻合。结论：光学相干断层扫描的分辨率和成本低，以及成像采集所需的探头的通用性使该光学技术成为诊断肾脏病理的一种有前景的手段。
Purpose: To determine histopathological status of living human kidneys in real time and a noninvasive fashion would be a significant advancement in renal disease diagnosis. Recently we reported that optical coherence tomography has the requisite high spatial resolution to noninvasively determine histopathological changes in rodent kidneys with mu m scale resolution. We established whether optical coherence tomography could 1) effectively penetrate the connective tissue capsule surrounding human kidneys, 2) provide a global survey of the human renal surface and 3) determine histopathological changes in human renal microstructure.Materials and Methods: Using a high speed optical coherence tomography system equipped with a frequency swept laser light source (1.3 mu m wavelength) we obtained cross-sectional images of 4 ex vivo human kidneys. All scanned sections underwent subsequent conventional light microscopic histological analysis, allowing direct comparisons.Results: Optical coherence tomography enabled characterization of the tubules, glomeruli and cortical vessels with a penetration depth of up to 2 mm and 10 pin spatial resolution. We surveyed and imaged an entire human kidney within minutes in a noninvasive fashion. Acquired optical coherence tomography images documented histopathological changes in the tubules, glomeruli and interstitium that closely matched the conventional histological observations.Conclusions: Optical coherence tomography resolution and low cost, and the versatility of the probes required for imaging acquisition make this optical technology a promising modality to diagnose renal pathology.