APPEARANCE OF DETERIORATED NEURONS ON REGIONALLY DIFFERENT TIME TABLES IN RAT-BRAIN THIN SLICES MAINTAINED IN PHYSIOLOGICAL CONDITION
APPEARANCE OF DETERIORATED NEURONS ON REGIONALLY DIFFERENT TIME TABLES IN RAT-BRAIN THIN SLICES MAINTAINED IN PHYSIOLOGICAL CONDITION
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DOI：
10.1016/0304-3940(94)11156-d
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发表时间：
1995-01-16
影响因子：
2.5
通讯作者：
NISHINO, H
中科院分区：
文献类型：
--
作者：
FUKUDA, A;CZURKO, A;NISHINO, H
脑切片广泛用于电生理实验。然而，时序细胞退化的时间表及其切片的区域差异是未知的。嗜银III染色法可以特异性地显示退化（“塌陷”）的神经元。因此，我们研究了外观的“崩溃”的神经元与时间在冠状脑“薄”切片保持在体外评估可能的区域差异的脆弱性在海马，CA 1锥体，齿状回颗粒，和非锥体细胞在任何子字段成为嗜银最容易，因为在新皮质层V-VI锥体神经元。这些结果表明，即使在生理条件下，脑切片中的某些神经元亚类也比其他神经元更早降解。
Brain slices are widely used for electrophysiological experiments. However, the time table of the chronological cell deterioration and its regional difference in slices are unknown. The argyrophil III staining method can demonstrate deteriorated ('collapsed') neurons specifically. Therefore we studied the appearance of the 'collapsed' neurons with time in coronal brain 'thin' slices maintained in vitro to evaluate possible regional differences in vulnerability In the hippocampus, CA1 pyramidal, dentate gyrus granule, and nonpyramidal cells in any subfield became argyrophilic most easily, as did layer V-VI pyramidal neurons in the neocortex. These results suggest that some subclasses of neurons in brain slices degrade earlier than others even when maintained in physiological condition.