Effects of antibiotics on enhanced biological phosphorus removal and its mechanisms
Effects of antibiotics on enhanced biological phosphorus removal and its mechanisms
复制标题
抗生素强化生物除磷效果及其机制
DOI：
10.1016/j.scitotenv.2021.145571
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发表时间：
2021
影响因子：
9.8
通讯作者：
Zou Xiaoming
中科院分区：
文献类型：
--
作者：
Wu Ligui;Wei Quantao;Zhang Yingying;Fan Yuxing;Li Mi;Rong Lingling;Xiao Xiaoyu;Huang Xiangfeng;Zou Xiaoming
多种抗生素不断排放到废水中，通常会导致污水处理性能大幅下降，而常用抗生素对磷酸盐去除影响的差异机制尚不清楚。因此，本研究开发了一种增强型生物除磷系统（EBPR）作为一种有效的除磷方法，并研究了其在短期（8 h）和长期（15 d）处理含抗生素人工废水中的性能。结果表明，抗生素的添加对除磷效果有显著抑制作用（P < 0.05）。为了解释这一现象，建立了机理方程，结果表明，在短期试验中，差异主要是由于抑制聚羟基烷酸酯（PHA）降解和多磷酸激酶（PPK）活性，从而导致对可溶性正磷（SOP）摄取过程的不同抑制。在长期试验中，SOP摄取的差异主要是由抑制PHA降解和PPK活性引起的，而SOP释放的差异是由抑制外多磷酸酶（PPX）和腺苷酸激酶（ADK）的活性引起的。此外，还从分子对接和静电势的角度确定了这种抑制的微观机制。
Many kinds of antibiotics are continuously discharged into wastewater and typically cause a great decrease in sewage treatment performance, whereas mechanisms of differences in the impacts of commonly used antibiotics on phosphate removal are still elusive. Thus, an enhanced biological phosphorus removal (EBPR) system, as an effective method of phosphate removal, was developed, and its performance in the treatment of artificial wastewater containing antibiotics at short- (8 h) and long-term (15 days) exposure was investigated. The results show that phosphorus removal was consistently inhibited by the addition of antibiotics with a significant difference (P < 0.05). To interpret the phenomena, mechanistic equations were developed, and the results indicate that for short-term tests, the difference was mainly caused by the suppression of polyhydroxyalkanoate (PHA) degradation and the activity of polyphosphate kinase (PPK), resulting in the different inhibition of the soluble orthophosphorus (SOP) uptake process. For long-term tests, the difference in SOP uptake was principally caused by the inhibition of PHA degradation and the activity of PPK, whereas the difference in SOP release resulted from the inhibition of activities of exopolyphosphatase (PPX) and adenylate kinase (ADK). Moreover, micro-mechanisms of such inhibition were identified from molecular docking and electrostatic potential.