Coimmobilization of L-alpha-glycerophosphate oxidase with catalase and its application for the synthesis of dihydroxyacetone phosphate
Coimmobilization of L-alpha-glycerophosphate oxidase with catalase and its application for the synthesis of dihydroxyacetone phosphate
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DOI：
10.1016/s0040-4020(97)01009-0
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发表时间：
1997-10-27
期刊：
影响因子：
2.1
通讯作者：
Steckhan, E
中科院分区：
文献类型：
--
作者：
Kramer, L;Steckhan, E
L-α-甘油磷酸（1）氧化成磷酸二羟丙酮（2）是在有氧条件下通过与过氧化氢酶共固定的L-α-甘油磷酸氧化酶酶促进行的，过氧化氢酶用于分解所产生的过氧化氢。2在与D/L-甘油醛的醛缩酶反应中被捕获（3）。共固定化酶显示出增加的操作稳定性，并且可以在进一步的转化中重复使用而不损失活性。(C)1997 Elsevier Science Ltd.
The oxidation of L-alpha-glycerophosphate (1) to dihydroxyacetone phosphate (2) is carried out enzymatically under aerobic conditions by L-alpha-glycerophosphate oxidase coimmobilized with catalase, which serves for the decomposition of the generated hydrogen peroxide. 2 is trapped in an aldolase reaction with D/L-glyceraldehyde (3). The coimmobilized enzymes show an increased operational stability and can be reused in further transformations without loss of activity. (C) 1997 Elsevier Science Ltd.