Optical activation of lateral amygdala pyramidal cells instructs associative fear learning
Optical activation of lateral amygdala pyramidal cells instructs associative fear learning
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DOI：
10.1073/pnas.1002418107
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发表时间：
2010-07-13
影响因子：
11.1
通讯作者：
LeDoux, Joseph E.
中科院分区：
文献类型：
--
作者：
Johansen, Joshua P.;Hamanaka, Hiroki;LeDoux, Joseph E.
Humans and animals can learn that specific sensory cues in the environment predict aversive events through a form of associative learning termed fear conditioning. This learning occurs when the sensory cues are paired with an aversive event occuring in close temporal proximity. Activation of lateral amygdala (LA) pyramidal neurons by aversive stimuli is thought to drive the formation of these associative fear memories; yet, there have been no direct tests of this hypothesis. Here we demonstrate that viral-targeted, tissue-specific expression of the light-activated channelrhodopsin (ChR2) in LA pyramidal cells permitted optical control of LA neuronal activity. Using this approach we then paired an auditory sensory cue with optical stimulation of LA pyramidal neurons instead of an aversive stimulus. Subsequently presentation of the tone alone produced behavioral fear responses. These results demonstrate in vivo optogenetic control of LA neurons and provide compelling support for the idea that fear learning is instructed by aversive stimulus-induced activation of LA pyramidal cells.
