IDENTIFICATION OF THE MAJOR PHOSPHOPROTEIN SECRETED BY MANY RODENT CELL-LINES AS 2AR/OSTEOPONTIN - ENHANCED EXPRESSION IN H-RAS-TRANSFORMED 3T3 CELLS
IDENTIFICATION OF THE MAJOR PHOSPHOPROTEIN SECRETED BY MANY RODENT CELL-LINES AS 2AR/OSTEOPONTIN - ENHANCED EXPRESSION IN H-RAS-TRANSFORMED 3T3 CELLS
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DOI：
10.1016/s0006-291x(88)80028-7
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发表时间：
1988-11-30
影响因子：
3.1
通讯作者：
DENHARDT, DT
中科院分区：
文献类型：
--
作者：
CRAIG, AM;NEMIR, M;DENHARDT, DT
2AR是一种由小鼠JB6表皮细胞分泌的肿瘤促进剂诱导蛋白，是大鼠骨桥蛋白或44kD骨磷蛋白的小鼠同源物。我们在此报道，2AR还与pp69有关，pp69是正常大鼠肾脏细胞分泌的一种主要磷蛋白。针对pp69和β-半乳糖苷酶-2AR融合蛋白制备的抗血清能够免疫沉淀相同的主要磷酸蛋白，其表观分子量为55-69kD，由几个大鼠和小鼠细胞株分泌。转人膀胱癌T24(H-ras)癌基因的NIH3T3细胞分泌蛋白和胞浆mRNA水平显著升高。这些结果以及Senger和他的同事的工作(癌症研究，45,5818-5823,1985)表明，通过转化多种哺乳动物成纤维细胞和上皮细胞而增加2AR/pp69/骨桥蛋白的分泌通常与肿瘤的发生有关。
2ar, a tumor promoter-inducible protein secreted by mouse JB6 epidermal cells, is the murine homolog of rat osteopontin, or 44 kD bone phosphoprotein. We report here that 2ar is also related to pp69, a major phosphoprotein secreted by normal rat kidney cells. Antisera raised against pp69 and against .beta.-galactosidase-2ar fusion proteins are able to immunoprecipitate the same major phosphoproteins, of apparent Mr 55-69 kD, secreted by several rat and mouse cell lines. The levels of secreted protein and cytoplasmic mRNA are dramatically elevated in NIH 3T3 cells transformed with the human bladder cancer T24 (H-ras) oncogene. These results and the work of Senger and colleagues (Cancer Res., 45, 5818-5823, 1985) imply that enhanced secretion of 2ar/pp69/osteopontin by transformation of a wide variety of mammalian fibroblasts and epithelial cells is often correlated with tumorigenicity.