A combined algorithm for genome-wide prediction of protein function
A combined algorithm for genome-wide prediction of protein function
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DOI：
10.1038/47048
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发表时间：
1999-11-04
期刊：
影响因子：
64.8
通讯作者：
Eisenberg, D
中科院分区：
文献类型：
--
作者：
Marcotte, EM;Pellegrini, M;Eisenberg, D
超过20个完全测序的基因组的可用性推动了发现蛋白质功能和相互作用的新方法的发展。在此，我们通过相关进化(1)、相关信使RNA表达模式(2)和结构域融合模式(3)对蛋白质进行分组，以确定酵母Saccharomyces cervisiae的6217种蛋白质之间的功能关系。使用这些方法，我们发现了93000多个功能相关酵母蛋白之间的成对链接。表征蛋白和未表征蛋白之间的联系允许将一般功能分配给2557个先前未表征的酵母蛋白中的一半以上。一个功能未知的蛋白质家族，其人类同源物与结肠癌和酵母朊病毒Sup35有关，给出了功能联系的例子。
The availability of over 20 fully sequenced genomes has driven the development of new methods to find protein function and interactions. Here we group proteins by correlated evolution(1), correlated messenger RNA expression patterns(2) and patterns of domain fusion(3) to determine functional relationships among the 6,217 proteins of the yeast Saccharomyces cervisiae. Using these methods, we discover over 93,000 pairwise links between functionally related yeast proteins. Links between characterized and uncharacterized proteins allow a general function to be assigned to more than half of the 2,557 previously uncharacterized yeast proteins. Examples of functional links are given for a protein family of previously unknown function, a protein whose human homologues are implicated in colon cancer and the yeast prion Sup35.