Matrine improves 2,4,6-trinitrobenzene sulfonic acid-induced colitis in mice
Matrine improves 2,4,6-trinitrobenzene sulfonic acid-induced colitis in mice
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DOI：
10.1016/j.phrs.2005.11.001
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发表时间：
2006-03-01
影响因子：
9.3
通讯作者：
Guo, QS
中科院分区：
文献类型：
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作者：
Cheng, H;Xia, B;Guo, QS
苦参碱是一种生物碱，主要存在于苦参、苦豆子、苦豆子等多种槐属植物中。目的：观察苦参碱对2，4，6-三硝基苯磺酸（TNBS）诱导的小鼠结肠炎的治疗作用。结肠灌注TNBS后2 h，连续7天灌胃给予不同剂量的苦参碱。与0.9%NaCl治疗的TNBS诱导的结肠炎小鼠相比，苦参碱（10和20 mg kg（-1））治疗的TNBS诱导的结肠炎小鼠的体重减轻，宏观评分，组织学评分和髓过氧化物酶（MPO）活性有所改善。此外，苦参碱（10和20 mg kg（-1））治疗降低了TNBS引起的肿瘤坏死因子-α（TNF-α）的mRNA和蛋白水平上调。我们的研究结果表明，苦参碱改善TNBS诱导的小鼠结肠炎，其治疗机制可能与减少TNBS引起的结肠TNF-α产生上调有关。(c)2005 Elsevier Ltd.保留所有权利。
Matrine is an alkaloid found in kinds of Sophora plants mainly including Sophora flavescens, Sophora alopecuroides and Sophora subprotrata. The aim of the present study was to evaluate therapeutic effects of matrine on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Two hours following colonic instillation of TNBS, matrine with several doses was given by gastric gavage once daily for 7 days. Comparing with the 0.9% NaCl-treated mice with TNBS-induced colitis, matrine (10 and 20 mg kg(-1))-treated mice with TNBS-induced colitis were shown improvements of weight loss, macroscopic score, histological score, and myeloperoxidase (MPO) activity. Moreover, treatments with matrine (10 and 20 mg kg(-1)) decreased the up-regulated mRNA and protein levels of tumour necrosis factor-alpha (TNF-alpha) caused by TNBS. Our findings suggest that matrine improves TNBS-induced colitis in mice and the therapeutic mechanism might be related to the reduction of up-regulated colonic TNF-alpha production caused by TNBS. (c) 2005 Elsevier Ltd. All rights reserved.