Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochlea
Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochlea
复制标题
DOI：
10.1016/s0378-5955(98)00206-8
复制
发表时间：
1999-02-01
期刊：
影响因子：
2.8
通讯作者：
Fujimoto, S
中科院分区：
文献类型：
--
作者：
Fujimura, T;Furukawa, H;Fujimoto, S
本文观察了成年雄性WBB6F(1)+/+小鼠和W/W-v突变体的血管纹中ET-1、ET-3、内皮素受体A型和Na，K-ATPase的免疫反应性。用包埋后的方法，在+/+小鼠体内，粗面内质网、高尔基体、胞浆小泡和溶酶体仅见于中间细胞的粗面内质网、高尔基体和溶酶体。野生型和突变型边缘细胞胞质膜上均有内皮素受体A的免疫反应，但突变型和野生型细胞的免疫反应性明显低于野生型和突变型。这些结果提示，在+/+小鼠血管纹功能的维持中，由中间细胞产生的内皮素可能起着一定的作用。由于W/W-v小鼠边缘细胞质膜对Na，K-ATPase也呈免疫反应，提示内皮素可能通过内皮素A受体参与工业边缘细胞钠泵的激活。此外，溶酶体在工业中间细胞吞噬内皮素中的作用也被提出。(C)1999 Elsevier Science B.V.保留所有权利。
Immunoreactivities of endothelin-1, endothelin-3, endothelin receptor type A, and Na,K-ATPase were investigated in the stria vascularis of adult male WBB6F(1) +/+ mice and in that of W/W-v mutants lacking strial intermediate cells. In the +/+ mice, electron microscopic immunoreactivity for the endothelins was seen on the rough endoplasmic reticulum, Golgi apparatus, cytoplasmic vesicles and lysosomes exclusively in the strial intermediate cells by the postembedment method. Immunoreactive endothelin receptor A was localized along the plasma membrane of strial marginal cells of both wild and mutant types although the immunoreactivity of the latter was much less than that of the former by the preembedment method. These findings suggest that the endothelins, which are produced in the strial intermediate cells, may play a role in the maintenance of the stria vascularis function in the +/+ mice. Since the plasma membrane of the marginal cells of the W/W-v mice, which do not generate a high positive endocochlear potential, also showed immunoreactivity for Na,K-ATPase, it seems likely that the endothelins are involved in the activation of sodium pump of the strial marginal cells by mediation of endothelin receptor A. In addition, the role of lysosomes in the crinophagy of the endothelins in the strial intermediate cells is proposed in the +/+ mice. (C) 1999 Elsevier Science B.V. All rights reserved.