The distribution of SIgA and IgG antibody-secreting cells in the palatine tonsils of Bactrian camels (Camelus bactrianus) of different ages.
The distribution of SIgA and IgG antibody-secreting cells in the palatine tonsils of Bactrian camels (Camelus bactrianus) of different ages.
复制标题
不同年龄双峰驼（Camelus bactrianus）腭扁桃体SIgA和IgG抗体分泌细胞的分布。
DOI：
10.14670/hh-11-808
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发表时间：
2017-05
影响因子：
2
通讯作者：
Wenhui Wang
中科院分区：
文献类型：
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作者：
Wangdong Zhanga;Xuefen Tan;Wanhong He;Wenhui Wang
分泌型免疫球蛋白A（SIgA）抗体分泌细胞（ASCs）是粘膜免疫的主要效应细胞，免疫球蛋白G（IgG）ASCs也与粘膜免疫有关。本研究旨在探讨这两个ASC群体在不同年龄双峰驼腭扁桃体中的分布。将18头双峰驼分为3个年龄组：青春期（3-5岁）、中年期（6-16岁）和老年期（17-20岁）。通过组织学和免疫组化技术观察腭扁桃体不同部位的SIgA和IgG ASCs，并对其密度进行统计学分析。结果显示，各年龄组的ASCs主要分布于网状隐窝上皮的上皮下，其次分布于复层鳞状上皮的上皮下，少量ASCs位于滤泡外区。三个区域的密度依次降低（P<0.05）。但同一地区SIgA型ASCs的密度显著高于IgG型ASCs（P<0.05），且两种ASCs的密度均随年龄增长而降低。结果证实，双峰驼腭扁桃体是产生SIgA ASCs的主要黏膜免疫器官，网状隐窝上皮的上皮下是SIgA和IgG ASCs定植和功能活动的主要区域。
Secretory immunoglobulin A (SIgA) antibody-secreting cells (ASCs) are the major effector cells of mucosal immunity, and immunoglobulin G (IgG) ASCs are also associated with mucosal immunity. This study aimed to explore the distribution of these 2 ASC populations in the palatine tonsils of Bactrian camels of different ages. Eighteen Bactrian camels were divided into the following three age groups: pubertal (3-5 years), middle-aged (6-16 years) and old (17-20 years). SIgA and IgG ASCs within different sites of the palatine tonsils were observed through histological and immunohistochemical techniques, and their densities were analyzed using statistical methods. The results from all age groups showed that both the SIgA and IgG ASCs were primarily distributed in the subepithelial compartments of the reticulated crypt epithelium and secondarily distributed in the subepithelial compartments of the stratified surface squamous epithelium, with a few ASCs located in the extrafollicular region. Their densities in these three areas were significantly decreased in turn (P<0.05). However, the densities of SIgA ASCs were significantly higher than IgG ASCs in the same regions (P<0.05), and the densities of both ASC populations decreased with age. The results confirmed that Bactrian camel palatine tonsils are the primary mucosal immune organ producing SIgA ASCs, and the subepithelial compartment of the reticulated crypt epithelium is the primary region for the colonization and functional activity of SIgA and IgG ASCs.