Real-Time PCR
Real-Time PCR
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DOI：
10.3109/9780203997352.224
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发表时间：
2005
影响因子：
7.3
通讯作者：
S. Bustin
中科院分区：
文献类型：
--
作者：
S. Bustin
实时聚合酶链式反应 (PCR) 使用荧光报告分子来监测 PCR 反应每个循环期间扩增产物的产生。这将 DNA 扩增和检测步骤结合到一种均质测定中，并且无需使用凝胶电泳来检测扩增产物。适当的数据分析和/或使用适当的化学方法也消除了用于扩增子鉴定的 Southern 印迹或 DNA 测序的需要。其简单性、特异性和灵敏度，加上其高通量潜力和不断引入的新化学物质、更可靠的仪器和改进的方案，使实时 PCR 成为 DNA 检测的基准技术。
The real-time polymerase chain reaction (PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the DNA amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect amplification products. Appropriate data analysis and/or use of apposite chemistries also eliminates the need for Southern blotting or DNA sequencing for amplicon identification. Its simplicity, specificity, and sensitivity, together with its potential for high throughput and the ongoing introduction of new chemistries, more reliable instrumentation, and improved protocols, has made realtime PCR the benchmark technology for the detection of DNA.