Enrichment and Characterization of an Autotrophic Ammonia-Oxidizing Archaeon of Mesophilic Crenarchaeal Group I.1a from an Agricultural Soil
Enrichment and Characterization of an Autotrophic Ammonia-Oxidizing Archaeon of Mesophilic Crenarchaeal Group I.1a from an Agricultural Soil
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DOI：
10.1128/aem.05787-11
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发表时间：
2011-12-01
影响因子：
4.4
通讯作者：
Rhee, Sung-Keun
中科院分区：
文献类型：
--
作者：
Jung, Man-Young;Park, Soo-Je;Rhee, Sung-Keun
土壤硝化作用是影响农业生产力和环境污染的重要过程。虽然已经描述了土壤中氨氧化菌的一个栽培代表，但其他代表值得表征。我们描述了一个氨氧化古菌（菌株MY 1）在一个高度富集的文化来自农业土壤。荧光原位杂交显微镜显示，经过2年的富集，培养物由>90%的古细菌细胞组成。16 S rRNA和古细菌amoA基因的克隆文库各自具有单一序列。PCR未检测到细菌amoA基因。[C-13]碳酸氢盐同化试验表明，化学计量的C-13掺入到特定的甘油二烷基甘油四醚。菌株MY 1福尔斯在遗传学上属于泉古菌群I.1a;与“海生亚硝化菌”的序列比较显示16 S rRNA和amoA基因的相似性为96.9%和89.2%。完整的生长测定表明，菌株MY 1是化能自养的，嗜温的（最适温度为25 ℃），嗜盐的（最适pH值为6.5至7.0），和非嗜盐的（最适盐度为0.2%至0.4%）。动力学呼吸测定表明，菌株MY 1对氨和氧的亲和力远高于氨氧化细菌（AOB）。温室气体N_2O在MY_1菌株培养物中的产量较低，但与土壤AOB的产量相当。我们建议将这一新的土壤氨氧化古菌命名为“朝鲜亚硝化古菌”。"
Soil nitrification is an important process for agricultural productivity and environmental pollution. Though one cultivated representative of ammonia-oxidizing Archaea from soil has been described, additional representatives warrant characterization. We describe an ammonia-oxidizing archaeon (strain MY1) in a highly enriched culture derived from agricultural soil. Fluorescence in situ hybridization microscopy showed that, after 2 years of enrichment, the culture was composed of >90% archaeal cells. Clone libraries of both 16S rRNA and archaeal amoA genes featured a single sequence each. No bacterial amoA genes could be detected by PCR. A [C-13] bicarbonate assimilation assay showed stoichiometric incorporation of C-13 into Archaea-specific glycerol dialkyl glycerol tetraethers. Strain MY1 falls phylogenetically within crenarchaeal group I.1a; sequence comparisons to "Candidatus Nitrosopumilus maritimus" revealed 96.9% 16S rRNA and 89.2% amoA gene similarities. Completed growth assays showed strain MY1 to be chemoautotrophic, mesophilic (optimum at 25 degrees C), neutrophilic (optimum at pH 6.5 to 7.0), and nonhalophilic (optimum at 0.2 to 0.4% salinity). Kinetic respirometry assays showed that strain MY1's affinities for ammonia and oxygen were much higher than those of ammonia-oxidizing bacteria (AOB). The yield of the greenhouse gas N2O in the strain MY1 culture was lower but comparable to that of soil AOB. We propose that this new soil ammonia-oxidizing archaeon be designated "Candidatus Nitrosoarchaeum koreensis."