Correlation between polymorphisms in ADSL and GARS-AIRS-GART genes with inosine 5'-monophosphate (IMP) contents in Beijing-you chickens
Correlation between polymorphisms in ADSL and GARS-AIRS-GART genes with inosine 5'-monophosphate (IMP) contents in Beijing-you chickens
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DOI：
10.1080/00071668.2010.508486
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Wen, J.
中科院分区：
文献类型：
--
作者：
Ye, M. H.;Chen, J. L.;Wen, J.
1.以90日龄北京油鸡（BJY）为材料，研究了腺苷酸琥珀酸裂解酶缺乏症（ADSL）基因和甘氨酰胺核糖核苷酸合成酶-氨基咪唑核糖核苷酸合成酶-甘氨酰胺核糖核苷酸转化酶（GARS-AIRS-GART）基因的单核苷酸多态性与肌苷酸（IMP）含量的关系。2. ADSL基因外显子2的单核苷酸多态性对IMP含量有影响。遗传ADSL正等位基因的个体，无论是纯合型还是杂合型，其胸肌IMP含量均高于不遗传者。对于GARS-AIRS-GART基因获得了类似的结果。在GARS-AIRS-GART基因的5'非翻译区（UTR）的标记也是显着的IMP值。遗传该位点正等位基因的基因型的鸡IMP含量明显高于遗传该位点负等位基因纯合子的鸡。4. ADSL和GARS-AIRS-GART之间的交互作用被检测到的测试群体的体重和肌肉产量等性状。这两个位点以相加的方式起作用。由于IMP是肉中最重要的风味成分之一，因此在这两个基因上开发的标记以及组合基因型可用作改良鸡肉品质的潜在分子标记。
1. The association of single nucleotide polymorphisms identified within the ADSL (adenylosuccinate lyase deficiency) gene and GARS-AIRS-GART (glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase) gene with the content of inosine 5'-monophosphate (IMP) was studied in a population of male Beijing-you (BJY) chickens slaughtered at 90 d of age. 2. A single nucleotide polymorphism in exon 2 of the ADSL gene had an effect on IMP content. Chickens inheriting the positive allele at ADSL, both homozygous and heterozygous genotypes, had a higher content of IMP in breast muscle than did individuals without it. 3. Similar results were obtained for the GARS-AIRS-GART gene. The marker at the 5' untranslated region (UTR) of the GARS-AIRS-GART gene was also significant for the IMP value. Chickens inheriting the genotypes with the positive allele at this locus had a much higher content of IMP than did those homozygous for the unfavourable one. 4. Interactions between ADSL and GARS-AIRS-GART were detected for such traits as body weight and muscle yields in the tested population. The two loci acted in an additive fashion. Because IMP is one of the most important flavour components in meat, markers developed at these two genes, as well as the combination genotypes, could be used as potential molecular markers for improving chicken quality.