Reentrant condensation of proteins in solution induced by multivalent counterions
Reentrant condensation of proteins in solution induced by multivalent counterions
复制标题
DOI：
10.1103/physrevlett.101.148101
复制
发表时间：
2008-10-03
影响因子：
8.6
通讯作者：
Schreiber, F.
中科院分区：
文献类型：
--
作者：
Zhang, F.;Skoda, M. W. A.;Schreiber, F.
溶液中的带负电荷的球状蛋白质在添加两个临界浓度C* 和C**（C* < C**）之间的三价抗衡离子时发生缩合。C** 以上的这种重入缩合行为是由多价阳离子和酸性残基之间的短程静电相互作用引起的，其机理不同于DNA的情况。小角X射线散射表明C* 和C** 附近的抗衡离子结合蛋白质之间的短程吸引。Monte Carlo模拟（在这些强静电耦合条件下）支持通过结合多价抗衡离子的表面侧链上的电荷的有效反转。
Negatively charged globular proteins in solution undergo a condensation upon adding trivalent counterions between two critical concentrations C* and C**, C* < C**. This reentrant condensation behavior above C** is caused by short- ranged electrostatic interactions between multivalent cations and acidic residues, mechanistically different from the case of DNA. Small-angle x-ray scattering indicates a short- ranged attraction between counterion-bound proteins near C* and C**. Monte Carlo simulations (under these strong electrostatic coupling conditions) support an effective inversion of charge on surface side chains through binding of the multivalent counterions.