Artemisia herba-alba essential oil from Buseirah (South Jordan): Chemical characterization and assessment of safe antifungal and anti-inflammatory doses
Artemisia herba-alba essential oil from Buseirah (South Jordan): Chemical characterization and assessment of safe antifungal and anti-inflammatory doses
复制标题
DOI：
10.1016/j.jep.2015.08.005
复制
发表时间：
2015-11-04
影响因子：
5.4
通讯作者：
Salgueiro, L.
中科院分区：
文献类型：
--
作者：
Abu-Darwish, M. S.;Cabral, C.;Salgueiro, L.
民族药理学相关性：Artemisia herba-alba阿索（英语：Artemisia herba-alba）是一种药用植物，自古以来就被许多文化广泛用于传统医学。它用于治疗炎症性疾病（感冒，咳嗽，支气管炎，腹泻），传染病（皮肤病，疥疮，梅毒）和其他（糖尿病，神经痛）。在约旦的传统医学中，这种植物被用作防腐剂和治疗皮肤病，疥疮，梅毒，发烧以及月经和神经disorders. Objective的研究：考虑到传统的医药用途和缺乏科学研究解决参与这些生物活动的细胞和分子的球员，本研究旨在揭示A.材料和方法：对银合欢阿索精油进行化学分析。采用水蒸气蒸馏法从白草地上部分中分离得到白草挥发油，并对其进行了气相色谱（GC）和气相色谱-质谱（GC-MS）联用分析。对酵母菌、皮肤癣菌和曲霉菌菌株进行了抗真菌活性（最小抑制浓度和最小致死浓度）评价。为了探索精油抗真菌作用的机制，使用白色念珠菌进行芽管抑制试验。采用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物（MTT）测定法和A.结果：白柏油中主要含有含氧单萜类化合物，即1，8-桉叶素（20. 1%）、β-侧柏酮（25. 1%）、α-侧柏酮（22. 9%）和樟脑（10. 5%）。在测试的真菌菌株中，油表现出对红色毛癣菌和絮状表皮癣菌的潜力，最小抑制浓度（MIC）和最小致死浓度（MCL）值为0.32 mg/mL，新型隐球菌的MIC为0.64 mg/mL。结果表明，该油对黄瓜芽管的形成有较强的抑制作用.浓度为0.16 mg/mL时，对白色念珠菌的抑制率约为90%。重要的是，精油显着抑制NO的产生诱发的LPS在没有细胞毒性的浓度高达1.25 μ L/mL的巨噬细胞和高达0.32 μ L/mL的小胶质细胞。此外，RAW 264.7巨噬细胞、BW 2小胶质细胞和HaCaT角质形成细胞中细胞活力的评价显示，在高达0.32 μ L/mL的浓度下没有细胞毒性。具有抗真菌和抗炎活性，并且对几种哺乳动物细胞类型没有有害影响的白草油。这些发现为A的药理活性提供了重要信息。草药白精油，特别是其抗真菌和抗炎治疗价值，从而证明和加强这种植物在传统医学中的使用。(c)2015爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacologic relevance: Artemisia herba-alba Asso ("desert wormwood" in English; "armoise blanche" in French; "shaih" in Arabic), is a medicinal and strongly aromatic plant widely used in traditional medicine by many cultures since ancient times. It is used to treat inflammatory disorders (colds, coughing, bronchitis, diarrhea), infectious diseases (skin diseases, scabies, syphilis) and others (diabetes, neuralgias). In Jordanian traditional medicine, this plant is used as antiseptic and against skin diseases, scabies, syphilis, fever as well as menstrual and nervous disorders.Aim of the study: Considering the traditional medicinal uses and the lack of scientific studies addressing the cellular and molecular players involved in these biological activities, the present study was designed to unveil the antifungal and anti-inflammatory activities of A. herba-alba Asso essential oil at doses devoid of toxicity to mammalian cells.Materials and methods: Chemical analysis of A. herba-alba essential oil isolated by hydrodistillation from aerial parts was carried out by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The antifungal activity (minimal inhibitory concentrations and minimal lethal concentrations) was evaluated against yeasts, dermatophyte and Aspergillus strains. In order to explore the mechanisms behind the anti-fungal effect of the essential oil, the germ tube inhibition assay was evaluated using Candida albicans. The assessment of cell viability was accomplished using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the in vitro anti-inflammatory potential of A. herba-alba oil at the periphery and central nervous system was evaluated by measuring nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated mouse macrophages and microglia, respectively.Results: Oxygen-containing monoterpenes are the main compounds of the oil, namely 1,8-cineole (20.1%), beta-thujone (25.1%), alpha-thujone (22.9%) and camphor (10.5%). Among the fungal strains tested, the oil demonstrated potential against Trichophyton rubrum and Epidermophyton floccosum, with minimal inhibitory concentration (MIC) and minimal lethal concentration (MCL) values of 0.32 mg/mL and Cryptococcus neoformans with MIC of 0.64 mg/mL. The oil revealed a strong inhibitory effect on germ tube formation in C. albicans with inhibition of filamentation around 90% at a concentration 0.16 mg/mL. Importantly, the essential oil significantly inhibited NO production evoked by LPS without cytotoxicity at concentrations up to 1.25 mu l/mL in macrophages and up to 0.32 mu L/mL in microglia. Furthermore, evaluation of cell viability in RAW 264.7 macrophages, BW2 microgliacells and HaCaT keratinocytes showed no cytotoxicity at concentrations up to 0.32 mu L/mL.Conclusions: It was possible to find appropriate doses of A. herba-alba oil with both antifungal and anti-inflammatory activities and without detrimental effects towards several mammalian cell types. These findings add significant information to the pharmacological activity of A. herba-alba essential oil, specifically to its antifungal and anti-inflammatory therapeutic value, thus justifying and reinforcing the use of this plant in traditional medicine. (c) 2015 Elsevier Ireland Ltd. All rights reserved.