Protective role of lactobacilli in Shigella dysenteriae 1-induced diarrhea in rats
Protective role of lactobacilli in Shigella dysenteriae 1-induced diarrhea in rats
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DOI：
10.1016/j.nut.2007.03.003
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发表时间：
2007-05-01
期刊：
影响因子：
4.4
通讯作者：
Devaraj, S. Niranjali
中科院分区：
文献类型：
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作者：
Moorthy, Guhapriya;Murali, Malliga Raman;Devaraj, S. Niranjali
目的：对乳酸菌的研究例证了它们在体外对抗各种肠道病原体的用途。然而，志贺氏菌感染过程中乳酸菌的体内作用尚未得到评估。本研究评价鼠李糖乳杆菌和嗜酸乳杆菌对志贺氏菌1型腹泻大鼠中性粒细胞浸润和脂质过氧化的影响。方法：将大鼠分为8组（每组6只）。诱导大鼠接受单次口服剂量的痢疾杆菌（12 X 10(8) 菌落形成单位 [cfu]/mL）。治疗组大鼠单独或联合口服鼠李糖乳杆菌（1×10(7)cfu/mL）或嗜酸乳杆菌（1×10(7)cfu/mL）4天，随后给予志贺氏菌。实验结束时，处死动物，进行碱性磷酸酶、髓过氧化物酶和抗氧化剂的活性测定以及脂质过氧化物的评估。除了对基质金属蛋白酶（MMP；MMP-2 和 MMP-9）活性进行明胶酶谱分析外，还进行了超氧化物歧化酶和过氧化氢酶的活性染色。将一部分肠组织固定在 10% 福尔马林中进行组织学研究。 结果：单独施用痢疾杆菌 I 会导致髓过氧化物酶、脂质过氧化、碱性磷酸酶水平升高，以及 MMP-2 和 MMP-9 表达，同时抗氧化剂水平降低。与患病组相比，用鼠李糖乳杆菌 (1 x 10(7) cfu/mL) 和嗜酸乳杆菌 (1 x 10(7) cfu/mL) 组合进行预处理可显着减弱这些变化。组织学观察与生化参数相关。结论：在志贺氏菌感染期间，与单独使用这些菌株相比，鼠李糖乳杆菌加嗜酸乳杆菌提供了更好的保护。 (C) 2007 Elsevier Inc. 保留所有权利。
Objective: Studies on lactic acid bacteria exemplify their use against various enteropathogens in vitro. Nevertheless, in vivo effects of Lactobacillus during Shigella infection have not been evaluated. The present study evaluated the effect of Lactobacillus rhamnosus and Lactobacillus acidophilus on neutrophil infiltration and lipid peroxidation during Shigella dysenteriae 1-induced diarrhea in rats.Methods: The rats were divided into eight groups (n = 6 in each group). Induced rats received single oral dose of S. dysenteriae (12 X 10(8) colony-forming units [cfu]/mL). Treated rats received L. rhamnosus (1 x10(7) cfu/mL) or L. acidophilus (1 x 10(7) cfu/mL) orally for 4 d, alone or in combination, followed by Shigella administration. At the end of the experimental period, animals were sacrificed and the assay of the activity of alkaline phosphatase, myeloperoxidase, and antioxidants and the estimation of lipid peroxides were performed. Activity staining of superoxide dismutase and catalase was done in addition to gelatin zymography for matrix metalloproteinase (MMP; MMP-2 and MMP-9) activity. A portion of the intestinal tissue was fixed in 10% formalin for histologic studies.Results: Administration of, S. dysenteriae I alone resulted in increased levels of myeloperoxidase, lipid peroxidation, alkaline phosphatase, and the expression of MMP-2 and MMP-9 with concomitant decrease in the antioxidant levels. Pretreatment with the combination of L. rhamnosus (1 x 10(7) cfu/mL) and L. acidophilus (1 x 10(7) cfu/mL) significantly attenuated these changes when compared with the diseased group. Histologic observations were in correlation with biochemical parameters.Conclusion: Lactobacillus rhamnosus plus L. acidophilus offered better protection when compared with individual treatment with these strains during Shigella infection. (C) 2007 Elsevier Inc. All rights reserved.