Cell proliferation patterns during development of the equine placenta
Cell proliferation patterns during development of the equine placenta
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DOI：
10.1530/jrf.0.1170143
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发表时间：
1999-09-01
期刊：
影响因子：
--
通讯作者：
Stewart, E
中科院分区：
文献类型：
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作者：
Gerstenberg, C;Allen, WR;Stewart, E
胎盘植入涉及母体和胎儿组织的大量生长和重组。在这项研究中，使用增殖标记 Ki-67 抗原的免疫组织化学定位来监测母马胎盘形成过程中的细胞分裂。子宫内膜活检是从怀孕第 14 天到第 26 天之间的八匹母马以及八匹接受过各种黄体酮和雌激素组合治疗的不发情母马中获得的。子宫内膜和胎膜样本取自 19 匹在妊娠第 30 天至 250 天之间怀有正常受孕母马的母马，以及三匹失败的超特异性驴中马妊娠的母马。子宫内膜浅层的增殖在妊娠第 18 天时增加，这种效果可以通过在长期（18-36 天）黄体酮给药的最后 6 天补充苯甲酸雌二醇来模拟。胎儿绒毛膜带细胞在妊娠第30-32天剧烈增殖，但在侵入子宫内膜后停止分裂，而尿囊绒毛膜滋养层细胞在第38天后显示有丝分裂活性增加。子宫内膜腔上皮仅在真上皮绒毛膜胎盘初生绒毛形成后以及第58-70天开始增殖。这种效应仅在胎盘进一步提前的怀孕角中可见。在妊娠后半期，大部分有丝分裂活动仅限于仍在生长的小子叶的外围。在驴马妊娠中，妊娠第 70 天时，子宫内膜淋巴细胞浸润严重和胎盘植入不良的区域，母体和胎儿上皮细胞的增殖率显着降低。这些结果为进一步研究影响侵入性和非侵入性胎盘的因素提供了基础。
Placentation involves considerable growth and reorganization of both maternal and fetal tissues. In this investigation, immunohistochemical localization of the proliferation marker Ki-67 antigen was used to monitor cell division during placentation in mares; Endometrial biopsies were obtained from eight mares between day 14 and day 26 of pregnancy and from eight anoestrous mares that had been treated with various combinations of progesterone and oestrogen. Samples of endometrium and fetal membranes were obtained from 19 mares carrying normal horse conceptuses between day 30 and day 250 of gestation and from three failing extraspecific donkey-in-horse pregnancies. Proliferation in the superficial strata of the endometrium was increased by day 18 of gestation and this effect could be mimicked by supplementing with oestradiol benzoate during the last 6 days of a prolonged period (18-36 days) of progesterone administration. Fetal chorionic girdle cells were proliferating vigorously at days 30-32 of gestation, but stopped dividing after they invaded the endometrium, while the trophoblast cells of the allantochorion showed an increase in mitotic activity after day 38. The luminal epithelium of the endometrium started to proliferate only after the primary villi of the true epitheliochorial placenta had been formed, and during days 58-70 this effect was seen only in the pregnant horn in which placentation was further advanced. During the second half of gestation, most of the mitotic activity was confined to the periphery of the microcotyledons which were still growing. Ln the donkey-in-horse pregnancies, proliferation rates of the maternal and fetal epithelia at day 70 of gestation were markedly reduced in areas of heavy endometrial lymphocyte infiltration and poor placentation. These results provide a basis for further studies on factors that influence invasive and non-invasive placentation.