Ataxic gait analysis in a mouse model of the olivocerebellar degeneration
Ataxic gait analysis in a mouse model of the olivocerebellar degeneration
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DOI：
10.1016/j.bbr.2010.01.035
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发表时间：
2010-06-26
影响因子：
2.7
通讯作者：
Vozeh, Frantisek
中科院分区：
文献类型：
--
作者：
Cendelin, Jan;Voller, Jaroslav;Vozeh, Frantisek
Lurcher突变小鼠代表了橄榄小脑变性的模型。出生后，浦肯野细胞完全丧失，颗粒细胞和下橄榄神经元继发性减少。小脑性共济失调是小脑退化的症状之一。这项工作的目的是确定 Lurcher 小鼠因功能性小脑剥皮引起的小脑共济失调而改变的步态参数，并评估步态参数、步行速度和旋转测试中表现之间的相关性。我们使用了 B6CBA 品系的成年 Lurcher 突变体和野生型小鼠。 CatWalk 系统用于步态分析。用转棒检查运动功能。数据分析显示，潜伏者和对照者在许多步态参数上存在显着差异。然而，几乎所有参数都与步行速度相关，并且在对步行速度进行校正后，差异消失了。问题是 Lurchers 的主要变化是什么——是步行速度还是个体步态参数。在转棒测试中，Lurcher 突变体的结果明显比野生型动物差。没有发现步态参数与转棒测试中的表现之间存在相关性。 (C) 2010 Elsevier B.V. 保留所有权利。
Lurcher mutant mice represent a model of olivocerebellar degeneration. Postnatally, a complete loss of Purkinje cells and secondary reduction of granule cells and inferior olive neurons occurs. Cerebellar ataxia is among the symptoms of degeneration of the cerebellum. The aim of the work was to identify gait parameters which are changed in Lurcher mice due to cerebellar ataxia arising from functional cerebellar decortication, and to assess the correlation between gait parameters, walking speed and performance in rotarod test. We used the adult Lurcher mutant and wild type mice of the B6CBA strain. For gait analysis the CatWalk system was used. Motor functions were examined with the rotarod. Data analysis revealed significant differences between Lurchers and controls in many gait parameters. However, almost all parameters correlated with the walking speed and the differences disappeared after the correction to the walking speed. The question is what is the primary change in Lurchers-whether the walking speed or individual gait parameters. In the rotarod test, the Lurcher mutants revealed significantly worse results than the wild type animals. No correlation between the gait parameters and performance in the rotarod test was found. (C) 2010 Elsevier B.V. All rights reserved.