Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules.
Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules.
复制标题
兔皮质集合管顶膜钙激活钾通道的单通道记录。
DOI：
10.1073/pnas.81.13.4237
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
Giebisch,GH
中科院分区：
文献类型：
--
作者：
Hunter,M;Lopes,AG;Boulpaep,EL;Giebisch,GH
用膜片钳技术记录了家兔皮质集合小管顶膜钾通道。通道的导电性能进行了研究，在由内而外的补丁。开放通道的斜率电导约等于90 pS。该通道对钾的选择性高于钠，选择性比为9：1。降低钙离子浓度的解决方案洗澡的细胞质面的补丁的结果在降低的开放通道的概率。将钙浓度降低至10 nM或更低完全抑制通道活性。该通道也被钡以剂量依赖性方式抑制。
Recordings of single potassium channels from the apical membrane of rabbit cortical collecting tubule have been achieved using the patch-clamp technique. The conductive properties of the channel have been studied in inside-out patches. The slope conductance of the open channel is approximately equal to 90 pS. The channel is selective to potassium over sodium, with a selectivity ratio of 9:1. Decreasing the calcium concentration of the solution bathing the cytoplasmic face of the patch results in a decrease of the open-channel probability. Decreasing the calcium concentration to 10 nM or less completely inhibited channel activity. The channel is also inhibited by barium in a dose-dependent fashion.