Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin
Overall-Mouth Disinfection by Photodynamic Therapy Using Curcumin
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DOI：
10.1089/pho.2011.3053
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发表时间：
2012-02-01
影响因子：
--
通讯作者：
Bagnato, Vanderlei Salvador
中科院分区：
文献类型：
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作者：
Araujo, Natalia Costa;Fontana, Carla Raquel;Bagnato, Vanderlei Salvador
背景资料：光动力疗法是一种在组织氧存在的情况下，通过光激活光敏剂，导致产生能够诱导细胞死亡的反应性自由基的技术。目的：研究姜黄素致敏和450 nm蓝光照射后唾液细菌对光动力疗法的总体敏感性。方法：对13名成年志愿者进行随机对照试验。分析3组：L-D-(无光，无药物；对照组)、L-D+(仅用药物治疗；姜黄素组)和L+D+(药物+光治疗；光动力疗法组)。在基线和实验阶段结束后，收集非刺激性唾液样本进行细菌计数，并记录所经历的不良事件。进行了一系列的稀释，所得到的样品在微需氧条件下培养在血液琼脂平板上。然后测定集落形成单位的数量。结果：试验组与对照组在细菌减少效果方面有显著差异。L-D+组细菌下降率(9%)明显小于L+D+组，细菌下降率为68%。只有光动力疗法组的细菌数量在统计学上显著减少(p<；0.05)。结论：光动力疗法能有效减少唾液微生物。仅使用姜黄素的那一组没有发现明显的减少，证明该药物没有暗毒性。这项工作表明，口腔的整体消毒可以通过一个涉及光动力作用的简单程序来完成。
Background data: Photodynamic therapy is a technique that involves the activation of photosensitizers by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. Objective: The present study assessed the overall susceptibility of pathogens of salivary flora to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. Methods: A randomized trial was executed with 13 adult volunteers. Three different groups were analyzed: L-D- (no light, no drug; control group), L-D+ (treated only with the drug; curcumin group) and L+D+ (treated with drug and light; photodynamic therapy group). Non-stimulated saliva samples were collected for bacterial counts at baseline and after the experimental phase, and adverse events experienced were recorded. Serial dilutions were performed, and the resulting samples were cultured on blood agar plates in microaerophilic conditions. The number of colony-forming units was then determined. Results: There was a considerable difference between the two experimental groups with regard to effectiveness of bacterial reduction. In the L-D+ group, the bacterial decline was considerably smaller (9%) than in the L+D+ group, with a 68% decrease in bacteria. A statistically significant reduction in the bacterial population was observed only in the photodynamic therapy group (p < 0.05). Conclusions: Photodynamic therapy was found to be effective in the reduction of salivary microorganisms. No significant reduction was found for the group in which only curcumin was used, proving the absence of dark toxicity of the drug. This work shows that overall disinfection of the mouth can be done with a simple procedure involving photodynamic action.