Enrichment of pluripotent hemopoietic progenitor cells from human bone marrow.
Enrichment of pluripotent hemopoietic progenitor cells from human bone marrow.
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富集人骨髓中的多能造血祖细胞。
DOI：
10.1182/blood.v64.4.774.774
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发表时间：
1984
期刊：
影响因子：
20.3
通讯作者：
M. Beard
中科院分区：
文献类型：
--
作者：
M. Bodger;I. Hann;R. Maclean;M. Beard
人骨髓多能造血祖细胞(CFU-GEMM，在甲基纤维素中形成混合造血祖细胞)通过使用单抗RFB-1在荧光激活细胞分选仪上进行阳性选择，扩增了90倍。根据细胞大小，用log90度光散射分离骨髓细胞，并用RFB-1抗原的相对荧光强度进一步分离含有CFU-GEMM的细胞部分。在RFB-1选择之前，通过耗尽骨髓中的T细胞和成熟的髓细胞，实现了进一步的浓缩，最高可达150倍。这些程序产生的细胞部分包含51%的原始细胞，2%的早幼粒细胞和47%的未分化(淋巴细胞样)单核细胞，尽管只有1%的细胞形成了混合集落。CFU-GEMM对RFB-1抗原呈强阳性，而形态可识别的红细胞、成髓细胞和早幼粒细胞呈弱RFB-1+。这表明，RFB-1抗原在骨髓细胞上的相对浓度与其成熟度呈负相关。用对数90度光散射分离骨髓后，CFU-GEMM的回收率大大提高，T细胞和成熟髓系细胞被去除，表明正常调节CFU-GEMM克隆效率的辅助细胞被去除。
Pluripotent hemopoietic progenitor cells (CFU-GEMM, cells forming mixed hemopoietic colonies in methylcellulose) from human bone marrow were enriched 90-fold by positive selection on the fluorescence-activated cell sorter using monoclonal antibody RFB-1. Bone marrow cells were separated by cell size, using log 90 degrees light scatter, and the cell fraction containing CFU-GEMM was further separated by relative fluorescence intensity for the RFB-1 antigen. Further enrichment, up to 150-fold, was achieved by depleting bone marrow of T cells and mature myeloid cells prior to RFB-1 selection. These procedures yield a cell fraction containing 51% blast cells, 2% promyelocytes, and 47% undifferentiated (lymphocyte-like) mononuclear cells, although only 1% of the cells formed a mixed colony. CFU-GEMM are strongly positive for the RFB-1 antigen, whereas morphologically identifiable erythroblasts, myeloblasts, and promyelocytes are weakly RFB-1+. This suggests that the relative concentration of the RFB-1 antigen on bone marrow cells is inversely related to their maturity. The greatly increased recovery of CFU-GEMM after the separation of bone marrow by log 90 degrees light scatter and the removal of T cells and mature myeloid cells suggested that accessory cells that normally regulate the cloning efficiency of CFU-GEMM were removed.
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通过密度梯度电泳分离人骨髓细胞群：骨髓细胞 (CFU-C)、单核细胞和淋巴细胞的迁移率差异。
影响因子：
4.3
作者：
Platsoucas,CD;Beck,JD;Kapoor,N;Good,RA;Gupta,S
通讯作者：
Gupta,S
鼠胎儿造血祖细胞的分离和各种红系前体细胞的选择性分级。
影响因子：
20.3
作者：
Nicola,NA;Metcalf,D;vonMelchner,H;Burgess,AW
通讯作者：
Burgess,AW
人多能造血干细胞（CFU-GEMM）的体外研究。
影响因子：
20.3
作者：
Ash,RC;Detrick,DA;Zanjani,ED
通讯作者：
Zanjani,ED
使用岩藻糖结合凝集素和细胞分选从人骨髓中纯化造血祖细胞。
影响因子：
20.3
作者：
Morstyn,G;Nicola,NA;Metcalf,D
通讯作者：
Metcalf,D
从人外周血中富集造血祖细胞（CFUC 和 BFUE）。
影响因子：
2.6
作者：
Wisniewski,D;Platsoucas,C;Strife,A;Lambek,C;Clarkson,B
通讯作者：
Clarkson,B