Isolation of Monosomics in Yeast
Isolation of Monosomics in Yeast
复制标题
酵母中单体的分离
作者：
J. Bruenn;R. Mortimer
在几个位点上的酿酒酵母三重体（A/A/A/a）的四倍体培养物形成孢子。所有的二倍体孢子预期是纯合的（A/A）或杂合的（A/a），因此具有显性表型。缺少一条染色体的细胞（单体）可能表现为隐性表型（a/−）。因此，认为在完全培养基上生长但在相关合成单缺失培养基上不能生长的孢子是单体的。这些分离物的进一步特点是孢子形成，并建立了几个稳定的单体。
Tetraploid cultures of Saccharomyces cerevisiae triplex (A/A/A/a) at several loci were sporulated. All the diploid spores are expected to be homozygous (A/A) or heterozygous (A/a) and, hence, to have the dominant phenotype. Cells lacking one of the chromosomes (monosomics) may show the recessive phenotype (a/−). Therefore, spores that grew on complete medium but failed to grow on the relevant synthetic single-omission media were presumed to be monosomic. These isolates were further characterized by sporulation, and several stable monosomics were established.