Spectroscopic studies on the interaction of calf thymus DNA with the drug levetiracetam.
Spectroscopic studies on the interaction of calf thymus DNA with the drug levetiracetam.
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DOI：
10.1016/j.saa.2012.05.045
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发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
N. Shahabadi;Saba Hadidi
中科院分区：
文献类型：
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作者：
N. Shahabadi;Saba Hadidi
用吸收、发射、圆二色谱、熔融温度曲线和粘度研究了天然小牛胸腺DNA与药物左乙拉西坦(LV)的相互作用。用分光光度法研究了左乙拉西坦与脱氧核糖核酸的相互作用，结果表明左乙拉西坦能与脱氧核糖核酸结合，其结合常数(Kb=(4.9±0.2)×103M−~1)与沟槽结合药物相当。与Hoechst 33258的竞争荧光研究表明，左乙拉西坦具有取代脱氧核糖核酸结合的Hoechst 33258的能力，这表明它与DNA结合在一起，与Hoechst 33258在沟槽结合方面存在强烈竞争。此外，该药物还可引起CT-DNA CD光谱的可检测变化以及其粘度的变化。测定了DNA-亚甲蓝(MB)络合物和LV-DNA-MB络合物的熔融温度(Tm)。最后，所有的实验结果都证明了小槽结合是主要的结合方式。
The interaction of native calf thymus DNA with the drug levetiracetam (LV) has been investigated by absorption, emission, circular dichroism, melting temperature (Tm) curves and viscosity studies. Spectrophotometric studies of the interaction of levetiracetam with DNA have shown that it can binds to CT-DNA and the DNA binding constant (Kb=(4.9±0.2)×103M−1) is comparable to groove binding drugs. Competitive fluorimetric studies with hoechst 33258 have shown that levetiracetam exhibit the ability to displace the DNA-bound hoechst 33258 indicating that it binds to DNA in strong competition with hoechst 33258 for the groove binding. Furthermore, the drug induces detectable changes in the CD spectrum of CT-DNA as well as changes in its viscosity. The values of melting temperature (Tm) of DNA–methylene blue (MB) complex and LV–DNA–MB complex were determined. Finally, all of the experimental results prove that the minor groove binding must be predominant.